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Zawiasa, Anna; Agnieszka Olejnik-Schmidt, 2025, "Antibiotic susceptibility profiles and genetic determinants of resistance in Listeria innocua isolated from food in Poland", https://doi.org/10.18150/ZTEIKS, RepOD, V1
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[ENG:]
A total of 15 files containing experimental data have been deposited in the database. These include: a file with information on the isolates, their origin, resistance profiles, and the presence of resistance genes (Data_on_isolates_resistance_profiles.csv); two files with inhibition zone diameters around antibiotic discs obtained for the first and second technical replicates (Data_inhibition_zones_1st_technical_replicate.csv and Data_inhibition_zones_2nd_technical_replicate.csv); four files containing microbiological culture images with inhibition zones for the first technical replicate (Images_of_inhibition_zones_1st_technical_replicate_1.zip, Images_of_inhibition_zones_1st_technical_replicate_2.zip, Images_of_inhibition_zones_1st_technical_replicate_3.zip, and Images_of_inhibition_zones_1st_technical_replicate_4.zip); five files with images for the second technical replicate (Images_of_inhibition_zones_2nd_technical_replicate_1.zip, Images_of_inhibition_zones_2nd_technical_replicate_2.zip, Images_of_inhibition_zones_2nd_technical_replicate_3.zip, Images_of_inhibition_zones_2nd_technical_replicate_4.zip, and Images_of_inhibition_zones_2nd_technical_replicate_5.zip); and three sets of files presenting agarose gel electrophoresis PCR results for the respective resistance genes (Electrophoresis_results_for_the_mecA_gene.zip, Electrophoresis_results_for_the_lnuA_and_lnuB_genes.zip, and Electrophoresis_results_for_the_cfr_optrA_and_poxtA_genes.zip). The files are provided in .csv, .jpg, and .png formats and were prepared using Microsoft Word and Microsoft Excel. The data represent the results of a study analyzing the antimicrobial susceptibility of 51 Listeria innocua isolates, originating from raw and processed meat products as well as from the meat-processing environment, to 18 selected antibiotics. Antimicrobial susceptibility was determined using the disc diffusion method, following CLSI (2020) guidelines. Isolates exhibiting resistance or intermediate susceptibility were further analyzed for the presence of resistance genes using PCR.
[PL:]
W bazie danych zdeponowano 15 plików zawierających dane eksperymentalne. Obejmują one: Plik z danymi dotyczącymi izolatów, ich pochodzenia, profili oporności oraz obecności genów oporności: Data_on_isolates_resistance_profiles.csv, Dwa pliki z wielkościami stref zahamowania wzrostu wokół krążków antybiotykowych, uzyskane dla pierwszego i drugiego powtórzenia technicznego: Data_inhibition_zones_1st_technical_replicate.csv i Data_inhibition_zones_2nd_technical_replicate.csv, Cztery pliki zawierające zdjęcia posiewów mikrobiologicznych ze strefami zahamowania wzrostu dla pierwszego powtórzenia technicznego: Images_of_inhibition_zones_1st_technical_replicate_1.zip, , Images_of_inhibition_zones_1st_technical_replicate_2.zip, Images_of_inhibition_zones_1st_technical_replicate_3.zip i Images_of_inhibition_zones_1st_technical_replicate_4.zip, Pięć plików ze zdjęciami dla drugiego powtórzenia technicznego: Images_of_inhibition_zones_2nd_technical_replicate_1.zip, Images_of_inhibition_zones_2nd_technical_replicate_2.zip, Images_of_inhibition_zones_2nd_technical_replicate_3.zip, , Images_of_inhibition_zones_2nd_technical_replicate_4.zip i Images_of_inhibition_zones_2nd_technical_replicate_5.zip, Trzy zestawy plików przedstawiających wyniki elektroforezy agarozowej PCR dla poszczególnych genów oporności: Electrophoresis_results_for_the_mecA_gene.zip, Electrophoresis_results_for_the_lnuA_and_lnuB_genes.zip i Electrophoresis_results_for_the_cfr_optrA_and_poxtA_genes.zip. Pliki zapisano w formatach .csv, .jpg oraz .png, a zostały przygotowane przy użyciu programów Microsoft Word oraz Microsoft Excel. Dane przedstawiają wyniki badań nad analizą wrażliwości 51 izolatów bakterii Listeria innocua, pochodzących z surowych i przetworzonych produktów mięsnych oraz ze środowiska zakładów przetwórstwa mięsa, na 18 wybranych antybiotyków. Wrażliwość oznaczano metodą krążkowo-dyfuzyjną, zgodnie z wytycznymi CLSI (2020). Izolaty wykazujące oporność lub wrażliwość pośrednią poddano dalszej analizie w kierunku obecności genów oporności z zastosowaniem techniki PCR.
Listeria innocua, antimicrobial resistance, antibiotic, resistance genes
2028-12-07
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