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Adamiak, Kamil; Sidoryk-Węgrzynowicz, Marta; Dąbrowska-Bouta, Beata; Sulkowski, Grzegorz; Strużyńska, Lidia, 2024, "Primary astrocytes as a cellular depot of polystyrene nanoparticles", https://doi.org/10.18150/P2WGQ0, RepOD, V2
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The aim of the study is to evaluate the cytotoxic potential of PS-NPs on in vitro cultured cells such as primary astrocytes, neurons and their co-cultures. The internalization and distribution of PS-NPs within cells was visualized using confocal microscopy and the fluorescence intensity derived from PS-NP was quantified. The cytotoxic effect was assessed using the MTT and LDH assays. Additionally, the expression of glial fibrillary acidic protein (GFAP) was analysed as a marker of astrocytic activation.
Polystyrene nanoparticles, Neurotoxicity, Cell viability, Glia activation, Cellular internalization
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