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Proteomic analysis of the cerebral cortex of 8-month-old male Bphl-/- and Bphl+/+ mice

Version 1.0

Bretes, Ewa, 2025, "Proteomic analysis of the cerebral cortex of 8-month-old male Bphl-/- and Bphl+/+ mice", https://doi.org/10.18150/ZZDNUX, RepOD, V1

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Description

[ENG:]

This file contains data regarding comparative proteomics of the cerebral cortex of 8-month-old male Bphl-/- (n=6) and Bphl+/+ (n=5) mice housed under standard conditions. Cortex protein isolation was performed at the Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, using TAEB buffer with 0.1% SDS. Disulfide bridges were then reduced using TCEP and blocked with MMTS. Proteins were digested with trypsin. Peptides were labeled with TMTpro 16-plex and fractionated in a reversed-phase system. Proteomic analysis was performed using an Evosep One coupled to an Orbitrap Exploris 480 mass spectrometer at the Mass Spectrometry Laboratory at the Institute of Biochemistry and Biophysics of the Polish Academy of Sciences in Warsaw. Samples from Bphl-/- mice are marked with tags 1, 3, 5, 7, 11, and 13, while those from Bphl+/+ mice are marked with tags 4, 6, 8, 10, and 12.

The CSV file contains data in the following columns: Protein ID, Gene names, Fasta headers, id, Student's T-test Significant BPHL_WT, Student's T-test q-value BPHL_WT, Student's T-test Difference BPHL_WT, Student's T-test Fold change BPHL_WT, Mol. weight [kDa], Q-value, MS/MS count, Razor + unique peptides TMT16plex, Unique peptides TMT16plex, Sequence coverage TMT16plex [%], TMT16plex signals.

[PL:]

Plik zawiera dane dotyczące porównania proteomów kory mózgowej 8-miesięcznych samców myszy Bphl-/- (n=6) i Bphl+/+ (n=5) hodowanych w standardowych warunkach. Izolację białek z kory mózgowej prowadzono w Katedrze Biochemii i Biotechnologii Uniwersytetu Przyrodniczego w Poznaniu, przy wykorzystaniu buforu TAEB z 0,1% SDS. Następnie mostki disiarczkowe były redukowane z wykorzystaniem TCEP i blokowane MMTS. Białka strawiono trypsyną. Peptydy znakowano za pomocą TMTpro 16-plex i frakcjonowano w układzie faz odwróconych. Przeprowadzono analizę w wykorzystaniem Evosep One połączonego ze spektrometrem masowym Orbitrap Exploris 480 w Środowiskowym Laboratorium Spektrometrii Mas w Instytucie Biochemii i Biofizyki Polskiej Akademii Nauk w Warszawie. Próbki pochodzące od myszy Bphl-/- są oznaczone znacznikami: 1, 3, 5, 7, 11 i 13, a pochodzące od myszy Bphl+/+: 4, 6, 8, 10 i 12.

Plik csv zawiera następujące dane w kolejnych kolumnach Protein ID, Gene names, Fasta headers, id, Student's T-test Significant BPHL_WT, Student's T-test q-value BPHL_WT, Student's T-test Difference BPHL_WT, Student's T-test Fold change BPHL_WT, Mol. weight [kDa], Q-value, MS/MS count, Razor + unique peptides TMT16plex, Unique peptides TMT16plex, Sequence coverage TMT16plex [%], TMT16plex signals.

Subject
Medicine, Health and Life Sciences
Keyword

biphenyl hydrolase-like protein (BPHL), homocysteine thiolactone (HTL), hiperhomocysteinemia (Hhcy), homocysteine (Hcy), Alzheimer’s disease (AD)

2028-09-14

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