Raw data from the research article: Inhibitor of bromodomain and extraterminal domain proteins decreases transcription of Cd33 in the brain of mice subjected to systemic inflammation; a promising strategy for neuroprotection by Grzegorz A. Czapski, Marta Matuszewska, Magdalena Cieślik, Joanna B. Strosznajder. Folia Neuropathologica, 2024, 62(2), 127-135. https://doi.org/10.5114/fn.2024.138140
These data were used to create Figures 1-3.
Figure 1 presents the microarray analysis of mRNA levels of 31 genes being genetic risk factors for Alzheimer’s disease (AD) in the hippocampus of male mice C57BL6/J 12 hours after intraperitoneal administration of LPS. Each data point represents a Log2(fold-change) for a specific probe - a mean from 4 animals.
Figure 2A presents the mRNA levels of Brd2, Brd3, and Brd4 genes in the mouse hippocampus 3 and 12 h after intraperitoneal lipopolysaccharide (LPS) injection. qPCR analysis.
Figure 2B presents the mRNA levels of the Cd33 gene in the mouse hippocampus 3 and 12 h after intraperitoneal injection of lipopolysaccharide (LPS) and/or JQ1, an inhibitor of BET proteins. qPCR analysis.
Figure 3A presents the mRNA level of the Cd33 gene in the mouse microglial BV2 cells after 12 h incubation in the presence of blood serum from control and lipopolysaccharide (LPS)-treated mice. Male mice C57BL6/J received intraperitoneal injection of saline or LPS, then after 12 h blood was collected, and serum was prepared. BV2 cells were incubated in the presence of JQ1 for 30 min, then mice blood serum was added and incubation was continued for 12 h. qPCR analysis.
Figure 3B presents the mRNA level of the Tnf gene in the mouse microglial BV2 cells after 12 h incubation in the presence of blood serum from control and lipopolysaccharide (LPS)-treated mice. Male mice C57BL6/J received intraperitoneal injection of saline or LPS, then after 12 h blood was collected, and serum was prepared. BV2 cells were incubated in the presence of JQ1 for 30 min, then mice blood serum was added and incubation was continued for 12 h. qPCR analysis.
Additional description: "readme.txt".
(2024-06-03)
