If you don't see your institution, add your dataset to the main dataverse named "RepOD".
Select the dataverse to which you want to add the new dataset:
You need to Sign In/Sign Up to add a dataset.
Share this dataset on your favorite social media networks.
Cieślak, Dorota; Pawlak, Piotr; Warzych-Plejer, Ewelina; Fakruzzaman, Md., 2026, "Microscopic images (.czi) of bovine blastocyst-stage embryos (day 7 post-fertilization) produced in vitro (IVM/IVF/IVC) and subjected to the TUNEL assay (DeadEnd™ Fluorometric TUNEL System)", https://doi.org/10.18150/YAKCOY, RepOD, V1
Learn about Data Citation Standards.
[ENG:]
The dataset contains microscopy images of bovine embryos at the expanding blastocyst stage, produced in vitro under standard IVC conditions, using oocytes matured in vitro (IVM) either under control conditions or in medium supplemented with n-6 linoleic acid (LA) and n-3 α-linolenic acid (ALA) at a 3:1 ratio (75 µM : 25 µM). The embryos were fixed and subjected to the TUNEL assay according to the manufacturer’s instructions. The specimens were evaluated using a confocal microscope (LSM 880 Airyscan; Zeiss), and microscopy images were acquired using the software provided by the microscope manufacturer. Two categories of data were obtained: the number of cells in each embryo, based on DAPI staining (total cell number, inner cell mass [ICM], and trophectoderm [TE]), and the number of apoptotic blastomeres, based on TUNEL staining. Blastomere numbers were calculated from 3D reconstructions generated for each blastocyst using ImageJ (v1.54i) and images acquired with Zen software (v2.6).
The collection includes three folders containing microscopic images of bovine blastocysts from three experimental groups analyzed for total cell number and the occurrence of apoptosis using the TUNEL assay. The groups were: Bovine blastocysts TUNEL C1 IVM DMSO (23 blastocysts), Bovine blastocysts TUNEL C4 IVM CON (33 blastocysts), and Bovine blastocysts TUNEL E1 IVM+FA (30 blastocysts).
[PL:]
Zbiór zawiera zdjęcia mikroskopowe zarodków bydła w stadium ekspandującej blastocysty pozyskanych in vitro (IVC w warunkach standardowych) z użyciem oocytów poddanych dojrzewaniu in vitro (IVM) w warunkach kontrolnych lub w medium suplementowanym kwasami tłuszczowymi n6 LA/n3 ALA w stosunku 3:1 (75µM: 25µM). Zarodki utrwalono i poddano procedurze TUNEL zgodnie z wytycznymi producenta. Preparaty oceniano pod mikroskopem konfokalnym (LSM 880 Airyscan; Zeiss) uzyskując zdjęcia mikroskopowe z użyciem oprogramowania dostarczonego przez producenta mikroskopu. Uzyskano 2 kategorie danych: liczbę komórek w zarodku (barwienie DAPI; całkowita, ICM, TE) oraz liczbę blastomerów apoptotycznych (barwienie TUNEL). Liczba blastomerów była kalkulowana na bazie rekonstrukcji 3D dla każdej blastocysty generowanych z użyciem programu ImageJ (v1.54i) i obrazów uzyskanych z użyciem Zen (v2.6).
Zbiór obejmuje trzy foldery ze zdjęciami mikroskopowymi blastocyst bydła 3 grup badawczych analizowanych pod kątem liczby komórek oraz występowania apoptozy (TUNEL) 1. Bovine blastocysts TUNEL C1 IVM DMSO (23 blastocysty), 2. Bovine blastocysts TUNEL C4 IVM CON (33 blastocysty) oraz 3. Bovine blastocysts TUNEL E1 IVM+FA (30 blastocyst).
.czi images, TUNEL assay, apoptosis, blastomere number
Fakruzzaman, Md., Warzych, E., Pawlak, P., Madeja, Z.E., Cieślak, A., Szkudelska, K., Łechtańska, J., Lechniak, D. Effect of IVM media supplementation with a blend of n6/n3 fatty acids on the quality of bovine oocytes and blastocysts. Theriogenology. Vol. 242. 7427 https://doi.org/10.1016/j.theriogenology.2025.117427 https://doi.org/10.1016/j.theriogenology.2025.117427 doi: 10.1016/j.theriogenology.2025.117427
Different licenses for individual files
Please select a file or files to be deleted.
The file(s) will be deleted after you click on the Delete button.
Files will not be removed from previously published versions of the dataset.
Please select a file or files to be edited.
For selected file(s) set a license to
Please select a file or files to be downloaded.
Please select a file or files for access request.
Please select restricted file(s) to be unrestricted.
You need to Log In/Sign Up to request access to this file.
Please confirm and/or complete the information needed below in order to continue.
Asterisks indicate required fields
Access to file(s) subject to additional consent under following conditions:
The restricted file(s) selected may not be downloaded because you have not been granted access.
Click Continue to download the files you have access to download.
Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.
Are you sure you want to lift the embargo?
Once you lift the embargo, you will not be able to set it again.
Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.
Use a Anonymized Private URL to allow those without Dataverse accounts to access your dataset. For more information about the Private URL feature, please refer to the User Guide.
Private URL has not been created.
"WARNING. This dataset has at least one published version. Those who have access to the Anonymized Private URL for this dataset may be able to use its accessible metadata to look up the full, not anonymized version of this dataset.
Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your dataset.
Use a Private URL to allow those without Dataverse accounts to access your dataset. For more information about the Private URL feature, please refer to the User Guide.
You will not be able to make changes to this dataset while it is in review.
This dataset cannot be published until Agriculture and Horticulture is published. Would you like to publish both right now?
Once you publish this dataset it must remain published.
Are you sure you want to republish this dataset?
Select if this is a minor or major version update.
This dataset cannot be published until Agriculture and Horticulture is published by its administrator.
This dataset cannot be published until Agriculture and Horticulture and Poznań University of Life Sciences are published.
Are you sure you want to deaccession? The selected version(s) will no longer be viewable by the public.
Please fill this out to prove you are not a robot.
