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Turlewicz-Podbielska, Hanna, 2025, "Processing fluid in the laboratory diagnostics of PRRSV infection and the impact of sample pooling", https://doi.org/10.18150/U4RXNA, RepOD, V1
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Results of ELISA test from boar sera and their corresponding processing fluids, and from gilt sera in the form of optical density and sample to positive ratio regarding the detection of antibodies against porcine reproductive and respiratory syndrome virus (PRRSV). Results of real-time reverse-transcription PCR test from boar sera and their corresponding processing fluids, and from gilt sera in the form of Cycle treshold regarding the detection of RNA of PRRSV. The impact of pooling of one processing fluid sample (low-, medium- and high-positive) with 4, 9, 19, 39 and 79 negative processing fluid samples on the ELISA results regarding PRRSV. The impact of pooling one processing fluid sample (low-, medium- and high-positive) with 9, 19, 39, 79, 159 and 319 negative processing fluid samples on the real-time reverse-transcription PCR results regarding PRRSV (Cycle treshold).
processing fluid, porcine reproductive and respiratory syndrome virus, laboratory diagnostics
Turlewicz-Podbielska, H., Dors, A., Pomorska-Mól, M. Evaluation of the utility of testicular-only processing fluid for porcine reproductive and respiratory syndrome virus diagnostics and the effect of sample pooling on the test results. Journal of Veterinary Research, 2025 https://doi.org/10.2478/jvetres-2025-0011 https://doi.org/10.2478/jvetres-2025-0011 doi: 10.2478/jvetres-2025-0011
CC BY - Creative Commons Attribution 4.0
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