1. General Information
- **Data Set Title**: Chitosan-and levan-based wound dressings crosslinked with dialdehyde levan: Structural and biological stability under climatic aging
- **Authors**:
- Principal investigator and contact person: Dorota Chełminiak-Dudkiewicz, Nicolaus Copernicus University in Torun, e-mail: dorotachd@umk.pl.
-Investigator: Miloslav Machacek, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Czech Republic, e-mail:machamil@faf.cuni.cz
-Investigator: Jolanta Dlugaszewska, Poznan University of Medical Sciences, e-mail: jdlugasz@wp.pl
-Investigator: Magdalena Wujak, Nicolaus Copernicus University in Torun, e-mail: mwujak@cm.umk.pl
-Investigator: Aleksander Smolarkiewicz-Wyczachowski, Nicolaus Copernicus University in Torun, e-mail: asmolarkiewicz@doktorant.umk.pl
- **Date of data collection**: year 2025.
- **Place of data collection**: Torun
- **Keywords***: Dialdehyde Levan; Polysacharides; Wound dressing materials
Data language**: English
- **Sources of funding**: National Science Center (NCN) competition: SONATA 18- UMO-2022/47/D/NZ7/01821.
2. Overview of data and files
-**abbreviations**:
- DhLev - dialdehyde levan
- CBD@CS - cannabis oil-chitosan-based sponge
- 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber
- 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber
- 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber
- 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber
- 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber
- 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber
- CBD@Lev - cannabis oil-levan-based sponge
- 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber
- 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber
- 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber
- 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber
- 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber
- 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
- **File List**:
Fig.1.tiff: Characterization of the dialdehyde levan. ATR-FTIR spectra and SEM images. Legends: Lev-levan; DhLev - dialdehyde levan
Fig.2. tiff: The sponges' appearance after 0–6 months of storage in a climate chamber. a) Chitosan-based sponge and b) Levan-based sponge. Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
Fig.3.tiff: A SEM images of a) CBD@CS sponges and b) CBD@Lev sponges after storage in the climate chamber. B Porosity and C WVTR changes of the sponges after 0–6 months of storage. Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
Fig.4.tiff: Release of CBD from CBD@CS and CBD@Lev sponges after different storage periods in a climate chamber. Each value represents the mean of three independent replicates (n = 3). Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
Fig.5.tiff: Characterization of the activity of the tested sponges after 0–6 months of storage in a climate chamber. Ani-inflammatory (A) and antioxidant (B) properties of the sponges; (C) swelling rate. Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
Fig.6.tiff: The protein adsorption on the polysaccharide sponges. Amount of fibrinogen (A) and HSA (B) per sponge area. ⁎,#,$,&,^,! $ indicate p < 0.05 when compared to the corresponding CBD@CS, 1 M-CBD@CS, 2 M-CBD@CS, 3 M-CBD@CS, 4 M-CBD@CS, 5 M-CBD@CS, and 6 M-CBD@CS and CBD@Lev, 1 M-CBD@Lev, 2 M-CBD@Lev, 3 M-CBD@Lev, and 4 M-CBD@Lev, respectively. Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
Fig.7.tiff: Analysis of the biodegradation properties (A) and antibcterial properties (B) of the sponges. Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
Fig.8.tiff: Biological properties of the tested sponges after 0–6 months of storage in a climate chamber. Cell viability of L929 and HDF cells (A) and hemolysis assay on the sponges (B). Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
Fig.9.tiff: Changes in the ATR-FTIR spectra of the chitosan (A) and levan (B) sponges after 0–6 months of storage. C Difference ATR-FTIR spectra of 6 M-CBD@CS and 6 M-CBD@Lev sponges. Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
Fig.10.tiff: Analysis of thermogravimetric properties of the sponges. Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
graphical abstract.tiff: graphical abstract. Legend: CBD@CS - cannabis oil-chitosan-based sponge 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber CBD@Lev - cannabis oil-levan-based sponge 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber
Raw data:
anti-inflammatory chitosan. txt: The anti-inflammatory properties were assessed by inhibiting the denaturation of bovine serum albumin (BSA) using the chitosan sponges prepared. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
anti-inflammatory levan.txt: The anti-inflammatory properties were assessed by inhibiting the denaturation of bovine serum albumin (BSA) using the prepared levan sponges. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
antioxidant chitosan.txt: The raw data on the anti-inflammatory properties of the chitosan sponges were determined by the DPPH scavenging test. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
Antioxidant levan.txt: TThe raw data on the anti-inflammatory properties of the levan sponges were determined by the DPPH scavenging test. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
chitosan L929.txt: Testing cytotoxicity against L929 cells on chitosan sponges. The test was performed using the MTT assay on a BioTek Instruments plate reader, Winooski, VT, USA. The test was performed by Dr. Magdalena Wujak from the Faculty of Pharmacy at Nicolaus Copernicus University in Toruń. The data were prepared using GraphPad Prism 10 software. Legend: L929 - mouse fibroblast cells; CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
cs hdf.txt: Testing cytotoxicity against HDF cells on chitosan sponges. The test was performed using the MTT assay on a BioTek Instruments plate reader, Winooski, VT, USA. The test was performed by Dr. Magdalena Wujak from the Faculty of Pharmacy at Nicolaus Copernicus University in Toruń. The data were prepared using GraphPad Prism 10 software. Legend: HDF - human fibroblast cells; CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
biodegradation levan.txt: The biodegradability of the levan-sponge materials was determined by monitoring their weight changes for 14 days. The data was prepared using GraphPad Prism 10. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
biodegradation chitosan.txt: The biodegradability of the chitosan-sponge materials was determined by monitoring their weight changes for 14 days. The data was prepared using GraphPad Prism 10. Legend: CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
fibrinogen cs.txt: The fluorescence method was used to determine the adsorption of fibrinogen (FB) onto chitosan sponges. Fluorescence spectra were recorded at various time points at 25 °C, ranging from 290 to 400 nm for HSA and from 300 to 500 nm for Fb, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285-400 nm, the scanning speed was 100 nm/min, and Em/Ex bandwidths were 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
fibrynogen levan.txt: The fluorescence method was used to determine the adsorption of fibrinogen (FB) onto levan sponges. Fluorescence spectra were recorded at various time points at 25 °C, ranging from 290 to 400 nm for HSA and from 300 to 500 nm for Fb, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285-400 nm, the scanning speed was 100 nm/min, and Em/Ex bandwidths were 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
hemoliza cs.tab: The ex vivo hemolytic activity of chitosan sponges was tested on human red blood cells from an anonymous donor (Approval of the Ethical Committee of the Faculty of Pharmacy in Hradec Králové, Charles University no. UKFaF/46675/2023). A study was performed by Miloslav Machacek from the Faculty of Pharmacy in the Czech Republic. It was measured using a Tecan Infinite 200M plate reader. The data was prepared using GraphPad Prism 10. Legend: CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
HSA cs.txt: The fluorescence method was used to determine the adsorption of human serum albumin (HSA) onto the chitosan sponges. Fluorescence spectra were recorded at various time points at 25 °C, ranging from 290 to 400 nm for HSA and from 300 to 500 nm for Fb, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285-400 nm, the scanning speed was 100 nm/min, and Em/Ex bandwidths were 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
HSA levan.txt: The fluorescence method was used to determine the adsorption of human serum albumin (HSA) onto the levan sponges. Fluorescence spectra were recorded at various time points at 25 °C, ranging from 290 to 400 nm for HSA and from 300 to 500 nm for Fb, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285-400 nm, the scanning speed was 100 nm/min, and Em/Ex bandwidths were 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
levan hemoliza.tab: The ex vivo hemolytic activity of levan sponges was tested on human red blood cells from an anonymous donor (Approval of the Ethical Committee of the Faculty of Pharmacy in Hradec Králové, Charles University no. UKFaF/46675/2023). A study was performed by Miloslav Machacek from the Faculty of Pharmacy in the Czech Republic. It was measured using a Tecan Infinite 200M plate reader. The data was prepared using GraphPad Prism 10. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
levan HDF.txt: Testing cytotoxicity against HDF cells on levan sponges. The test was performed using the MTT assay on a BioTek Instruments plate reader, Winooski, VT, USA. The test was performed by Dr. Magdalena Wujak from the Faculty of Pharmacy at Nicolaus Copernicus University in Toruń. The data were prepared using GraphPad Prism 10 software. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
levan L929.txt: Testing cytotoxicity against L929 cells on levan sponges. The test was performed using the MTT assay on a BioTek Instruments plate reader, Winooski, VT, USA. The test was performed by Dr. Magdalena Wujak from the Faculty of Pharmacy at Nicolaus Copernicus University in Toruń. The data were prepared using GraphPad Prism 10 software. Legend: L929 - mouse fibroblast cells; CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
microbiological Cs.txt: Study of antimicrobial properties of chitosan sponges against S. aureus. A study was performed by Dr. Jolanta Długaszewska from the Faculty of Pharmacy at the Medical University of Poznań. The analysis was carried out by culturing bacterial strains in BHI medium containing the material. The data was prepared using GraphPad Prism 10. List of abbreviations: BHI - Bacterial Growth Medium; CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
pecznienie chitozan.tab: A swelling test was performed at pH 5.6 and pH 7.4 on the chitosan sponges. The samples were weighed, and the degree of swelling was calculated using a formula. The data was prepared using GraphPad Prism 10. Legend: CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
pecznienie levan.txt: A swelling test was performed at pH 5.6 and pH 7.4 on the levan sponges. The samples were weighed, and the degree of swelling was calculated using a formula. The data was prepared using GraphPad Prism 10. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
porowatosc chitozan.tab: The porosity of the chitosan sponges was characterized using an ethanol displacement method. The data was prepared using GraphPad Prism 10. Legend: CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
porowatosc lewan.tab: The porosity of the levan sponges was characterized using an ethanol displacement method. The data was prepared using GraphPad Prism 10. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
WVTR chitozan.tab: Measurement of water vapor transmission rate (WVTR) was used to estimate the chitosan sponges' ability to facilitate gas exchange. The data was prepared using GraphPad Prism 10. Legend: CBD@CS - cannabis oil-chitosan-based sponge; 1M-CBD@CS - cannabis oil-chitosan-based sponge after 1 month storage in a climate chamber; 2M-CBD@CS - cannabis oil-chitosan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@CS - cannabis oil-chitosan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@CS - cannabis oil-chitosan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@CS - cannabis oil-chitosan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@CS - cannabis oil-chitosan-based sponge after 6 months of storage in a climate chamber;
WVTR levan.tab: Measurement of water vapor transmission rate (WVTR) was used to estimate the chitosan sponges' ability to facilitate gas exchange. The data was prepared using GraphPad Prism 10. Legend: CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
microbiologia lewan.txt: Study of antimicrobial properties of chitosan sponges against S. aureus. A study was performed by Dr. Jolanta Długaszewska from the Faculty of Pharmacy at the Medical University of Poznań. The analysis was performed by culturing bacterial strains in BHI medium supplemented with the material. The data was prepared using GraphPad Prism 10. List of abbreviations: BHI - Bacterial Growth Medium; CBD@Lev - cannabis oil-levan-based sponge; 1M-CBD@Lev - cannabis oil-levan-based sponge after 1 month of storage in a climate chamber; 2M-CBD@Lev - cannabis oil-levan-based sponge after 2 months of storage in a climate chamber; 3M-CBD@Lev - cannabis oil-levan-based sponge after 3 months of storage in a climate chamber; 4M-CBD@Lev - cannabis oil-levan-based sponge after 4 months of storage in a climate chamber; 5M-CBD@Lev - cannabis oil-levan-based sponge after 5 months of storage in a climate chamber; 6M-CBD@Lev - cannabis oil-levan-based sponge after 6 months of storage in a climate chamber;
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(2025-06-22)
