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Szczepaniak, Oskar, 2025, "DNA interaction with thiopolysulfides and/or cell cycle regulators", https://doi.org/10.18150/MKHI1B, RepOD, V1
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[ENG:]
The database contains voltammograms recorded using square-wave voltammetry (SWV) for a synthetic, double-stranded oligonucleotide with the following sequence 5'GTCAACTTCCGTACCGAGC, that was electrostatically deposited on the surface of a carbon paste working electrode, and then interacted with various concentrations of diadenosine triphosphate (Ap3A)/diadenosine tetraphosphate (Ap4A) and/or allicin/S-allylcysteine. Detailed information on the methods, sequence of interactions, and compound concentrations can be found in the "Read me" file.
The data were recorded using a three-electrode system consisting of a working electrode – a carbon paste electrode, an AgCl reference electrode and a measuring electrode Pt. Double-stranded DNA was deposited on the outer layer of the working electrode using the +0.5V potential.
The files contain recorded signals in the form of current [μA] as a function of the gradually changing potential [V], corrected against the base signal – the background of the phosphate buffer used for research.
[PL:]
Baza zawiera woltamperogramy zarejestrowane techniką woltamperometrii fali prostokątnej (SWV) dla syntetycznego, dwuniciowego oligonukleotydu o sekwencji 5’GTCAACTTCCGTACCGAGC osadzonego elektrostatycznie na powierzchni elektrody pracującej za pasty węglowej, a następnie poddanego oddziaływaniu z diadenozynotrifosforanem (Ap3A)/diadenozynotetrafosforanem (Ap4A) i/lub allicyną/S-allilocysteiną o różnych stężeniach. Szczegółowe informacje na temat sposobów, kolejności oddziaływań oraz stężeń związków można znaleźć w pliku „Read me”.
Dane zarejestrowano przy pomocy układu trójelektrodowego składającego się z elektrody pracującej – elektrody z pasty węglowej, elektrody referencyjnej AgCl oraz elektrody pomiarowej Pt. Dwuniciowe DNA osadzano na powierzchni elektrody pracującej przy pomocy potencjału +0,5V.
Pliki zawierają zarejestrowane sygnały w postaci natężenia prądu [μA] jako funkcję stopniowo zmieniającego się potencjału [V], skorygowane o sygnał bazowy – tło buforu fosforanowego używanego do badań.
DNA biosensor, allicin, S-allylthiocysteine, Ap3A, Ap4A
2026-12-31
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