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Studzińska, Sylwia; Ostrowska, Karolina, 2026, "Antisense oligonucleotide extraction using commercial adsorbents", https://doi.org/10.18150/LZMJ0F, RepOD, V1
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The repository contains data on the development of a new mixed‑mode extraction of antisense oligonucleotides using a commercially available adsorbents. Four sorbents with various functional groups were systematically evaluated to elucidate their interaction with antisense oligonucleotides. As a result, dispersive solid‑phase extraction procedures were developed with adsorption and desorption controlled by electrostatic, hydrogen bonding, π-π, and hydrophobic interactions. Elution conditions were optimized using a central composite design (CCD), demonstrating that efficient desorption requires simultaneous modulation of salt pH and concentration, and organic solvent content. Two silica-based sorbents modified with phenylboronic acid and 2‑(2‑pyridyl)ethyl groups exhibited the most favorable balance of recovery, reproducibility, and sorption capacity, achieving recoveries up to 93%. Kinetic studies revealed rapid adsorption for both sorbents, while differences in desorption influenced method transferability from dispersive solid phase extraction to solid‑phase extraction. Finally, the extraction procedure for phenylboronic acid-based material enabled one‑step isolation of antisense oligonucleotides from serum without prior protein removal. Although substantial (1:5 v/v) serum dilution was required and recoveries were moderate (59-71%), the approach represents an alternative to currently used methods.
antisense oligonucleotides, solid phase extraction, commercial adsorbents, central composite design, recovery
CC BY - Creative Commons Attribution 4.0
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