1. General Information
- **Data Set Title**: Multifunctional laminarin-chitosan composites loaded with bromelain nanoflowers: a biohybrid design approach
- **Authors**:
- Principal investigator and contact person: Dorota Chełminiak-Dudkiewicz, Nicolaus Copernicus University in Torun, e-mail: dorotachd@umk.pl.
-Investigator: Miloslav Machacek, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Czech Republic, e-mail:machamil@faf.cuni.cz
-Investigator: Klara Konecna, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Czech Republic, e-mail:konecna@faf.cuni.cz
-Investigator: Monika Wujec, Medical University of Lublin, e-mail: monika.wujec@umlub.pl
- **Date of data collection**: year 2025.
- **Place of data collection**: Torun
- **Keywords***: Bromelain nanoflowersLaminarinLaminarin dialdehydeMannich base, antimicrobial propertiesBiohybrid wound care materialsContact-active biomaterial
Data language**: English
- **Sources of funding**: National Science Center (NCN) competition: SONATA 18- UMO-2022/47/D/NZ7/01821.
2. Overview of data and files
-**abbreviations**:
- BNFs - bromelain nanoflowers
- DhLam- dialdehyde laminarin
- MB - Mannich's base 4-(3-chlorophenyl)-5-(3-fluorophenyl)-2-[4-(4-nitrophenyl)piperazin-1-yl)methyl]-2,4-dihydro-3H-1,2,4-triazole-3-thione
- CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin
- CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
- **File List**:
Fig.1.tiff: The ATR-FTIR spectrum (A) and SEM image of the MB (B). Legend: MB - Mannich's base 4-(3-chlorophenyl)-5-(3-fluorophenyl)-2-[4-(4-nitrophenyl)piperazin-1-yl)methyl]-2,4-dihydro-3H-1,2,4-triazole-3-thione.
Fig.2. tiff: Characterization of the BNFs. (A) optical microscope image at various magnifications, (B) SEM images at various magnifications, (C) single structure resembling a Dahlia variabilis, dinner plate type flower, (D) single structure resembling a pompon-type Dahlia variabilis flower, and (E) 3D and roughness profile. Legend: BNFs-bromelain nanoflowers
Fig.3.tiff: Schematic illustration of the preparation of CS-Lam@BNF/MB sponge. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
Fig.4.tiff: Characterization of the obtained sponges. ATR-FTIR spectra (A), thermogravimetric analysis (B; TG – thermogravimetric curve showing weight loss; DTG – derivative thermogravimetric curve showing the rate of decomposition), SEM images of the CS-Lam (C) and CS-Lam@BNF/MB (D), surface roughness of the CS-Lam (E) and CS-Lam@BNF/MB (F), and porosity (G). Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
Fig.5.tiff: Mechanical properties (A), adhesion force (B), swelling rate (C), biodegradation properties (D), antioxidant (E) and anti-inflammatory (F) properites of the obtained sponges. Data are expressed as mean ± SD (n = 3). Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test. Significance levels: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****). Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
Fig.6.tiff: Protein adsorption on the surface of obtained sponges: (A) fibrinogen (Fb) and (B) human serum albumin (HSA). Data are expressed as mean ± SD (n = 3). Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test. Significance level: p < 0.05 (*). Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
Fig.7.tiff: Biological properties of the sponges. Antimicrobial analysis (A), cytotoxicity (B), and hemolysis assay (C). Statistical significance is indicated as follows: ns = not significant, p < 0.05 (*), p < 0.001 (***), p < 0.0001 (****). Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
Fig.8.tiff: Scratch-wound healing assay results showing fibroblast migration either in the presence of medium (control; yellow) or CS–Lam (black), and CS–Lam@BNF/MB (purple) sponges. Quantitative analysis of wound closure is presented as means (SD), n = 6 (A), and representative microscopic images of the wound areas at 0, 48, and 96 h are shown for each sample (B). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.). Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
graphical abstract.tiff: graphical abstract. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
Raw data:
adhezja. txt: The adhesive strength of the sponges on the glass, rubber, and metal surfaces was tested using a texture analyzer (EZ-Test E2-LX, Shimadzu, Kyoto, Japan) at room temperature. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
antiinflammatory.txt: The anti-inflammatory properties were assessed by inhibiting the denaturation of bovine serum albumin (BSA) using the materials prepared. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
antioxidant.txt: The raw data on the anti-inflammatory properties of the sponges were determined by the DPPH scavenging test. The results were recorded on a UV-1800 spectrophotometer. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
fibrinogen adsorption.txt: The fluorescence method was used to determine the adsorption of fibrinogen (FB) onto chitosan sponges. Fluorescence spectra were recorded at various time points at 25 °C, ranging from 290 to 400 nm for HSA and from 300 to 500 nm for Fb, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285-400 nm, the scanning speed was 100 nm/min, and Em/Ex bandwidths were 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
biodegradation.txt: The biodegradability of the sponges was determined by monitoring their weight changes for 14 days. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
hsa adsorption.txt: The fluorescence method was used to determine the adsorption of human serum albumin (HSA) onto the sponges. Fluorescence spectra were recorded at various time points at 25 °C, ranging from 290 to 400 nm for HSA and from 300 to 500 nm for Fb, with an excitation wavelength of 280 nm, using a Jasco FP-8300 spectrofluorometer (Jasco, Tokyo, Japan). The spectral registration range was 285-400 nm, the scanning speed was 100 nm/min, and Em/Ex bandwidths were 2.5 nm/5 nm. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
mechaniczne.tab: The mechanical properties of the sponges were tested using a texture analyzer (EZ-Test E2-LX, Shimadzu, Kyoto, Japan) at room temperature. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
microbiologia candida.txt: Study of antimicrobial properties of the sponges against Candida albicans. A study was performed by Dr. Klara Konecna from the Faculty of Pharmacy at Charles University in Hradec Králové. The analysis was performed by culturing bacterial strains in BHI medium supplemented with the material. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
mikrobiologia pseudomonas.txt: Study of antimicrobial properties of the sponges against Pseudomonas aureginosa. A study was performed by Dr. Klara Konecna from the Faculty of Pharmacy at Charles University in Hradec Králové. The analysis was performed by culturing bacterial strains in BHI medium supplemented with the material. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
mikrobiologia staphyloccous.txt: Study of antimicrobial properties of the sponges against Staphylococcus aureus. A study was performed by Dr. Klara Konecna from the Faculty of Pharmacy at Charles University in Hradec Králové. The analysis was performed by culturing bacterial strains in BHI medium supplemented with the material. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
porowatosc.txt: The sponges' porosity was characterized using an ethanol-displacement method. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
swelling.tab: A swelling test was performed at pH 5.6 and pH 7.4 on the sponges. The samples were weighed, and the degree of swelling was calculated using a formula. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
24h komorki.txt: Study of cytotoxicity properties against MRC cells after 24 hours of incubation. A study was conducted by Dr. Miloslav Machacek from the Faculty of Pharmacy in the Czech Republic. Fluorescence (λex = 560 nm, λem = 590 nm) was measured using the Tecan Infinite 200M plate reader (Tecan Group Ltd., Männedorf, Switzerland). The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
72h komorki.txt: Study of cytotoxicity properties against MRC cells after 72 hours of incubation. A study was conducted by Dr. Miloslav Machacek from the Faculty of Pharmacy in the Czech Republic. Fluorescence (λex = 560 nm, λem = 590 nm) was measured using the Tecan Infinite 200M plate reader (Tecan Group Ltd., Männedorf, Switzerland). The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
hemoliza.tab: The ex vivo hemolytic activity of the sponges was tested on human red blood cells from an anonymous donor (Approval of the Ethical Committee of the Faculty of Pharmacy in Hradec Králové, Charles University no. UKFaF/46675/2023). A study was performed by Miloslav Machacek from the Faculty of Pharmacy in the Czech Republic. It was measured using a Tecan Infinite 200M plate reader. The data was prepared using GraphPad Prism 10. Legend: extract PC-positive control NC-negative control; CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
scratch.txt: Wound healing potential was evaluated using a scratch assay on MRC-5 fibroblasts, with wound closure monitored for 72 h and quantified in ImageJ. The data was prepared using GraphPad Prism 10. Legend: CS-Lam - chitosan-laminarin sponge crosslinked with dialdehyde laminarin; CS-Lam@BNF/MB - chitosan-laminarin sponge crosslinked with dialdehyde laminarin, enriched with bromelain nanoflowers and Mannich’s base
- **File formats**: tiff, tab, txt
3. sharing and access information
- **Licenses/Restrictions**: CC BY - Creative Commons Attribution 4.0.
- **Related Publications**: https://www.sciencedirect.com/science/article/pii/S0264127525017952
(2025-12-23)
