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Hryhorowicz, Magdalena; Grześkowiak, Bartosz; Lipiński, Daniel; Olejnik, Przemysław; Zeyland, Joanna, 2024, "Data to article - Magnetic nanoparticles as a tool to increase the efficiency of CRISPR/Cas9 delivery in RNA format into porcine primary fibroblasts", https://doi.org/10.18150/FNTBD7, RepOD, V1
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The data concern the use of magnetic nanoparticles to delivery CRISPR/Cas9 in RNA format into porcine fibroblast cells in vitro. The levels of editing efficiency at the target site for pools of cells, measured as indel frequencies, were quantifed by TIDE (Tracking of Indels by DEcomposition) web tool. The cytotoxicity of magnetic nanoparticles was assessed using the CCK-8 assay (Cell Counting Kit-8 Assay) and the ELx808 plate reader (BioTek). Microscopic assessment of pig fibroblast cells after magnetofection and lipofection was performed using an Axiovert 200 inverted microscope.
magnetic nanoparticles, CRISPR/Cas9 delivery, magnetofection, genome editing, transfection
2027-05-29
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