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Gene expression NHX1 and SOS1 in Salicornia under different salinty levels

Version 1.0

Cardenas Perez, Stefany, 2024, "Gene expression NHX1 and SOS1 in Salicornia under different salinty levels", https://doi.org/10.18150/2NI8WA, RepOD, V1

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Description

The expression of the S. europaea genes NHX1 and SOS1 in different saline treatments was analysed using quantitative reverse transcriptase polymerase chain reaction (RT–qPCR). The analysed NHX1 and SOS1 genes encode a tonoplast Na+/H+ antiporter and a plasma membrane Na+ /H+ antiporter, respectively. The data contained in this file correspond to S. europaea population collected in Inowrocław, Poland


GENERAL INFORMATION


Title of Dataset: Salicornia qPCR SOS1 and NHX1


Author/Principal Investigator Information

Name: Stefany Cardenas Perez

ORCID:https://orcid.org/0000-0003-3919-3498

Institution: Department of Geobotany and Landscape Planning, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun

Address: Lwowska 1, 87-100 Torun, Poland 

Email: cardenasperez@umk.pl


Co-investigator Information

Name: Agnieszka Piernik

ORCID:https://orcid.org/0000-0002-0800-5266

Institution: Department of Geobotany and Landscape Planning, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun


Address: Lwowska 1, 87-100 Torun, Poland 

Email: piernik@umk.pl


Date of data collection: 02.2023 


Geographic location of data collection: Poland


Information about funding sources that supported the collection of the data: National Science Center 2021/43/D/NZ8/01137


SHARING/ACCESS INFORMATION


Licenses/restrictions placed on the data: CC0



DATA & FILE OVERVIEW


1. Salicornia qPCR SOS1 and NHX1


METHODOLOGICAL INFORMATION


The reverse transcription reaction, the RNA was treated with DNaseI (Thermo Fisher Scientific, Waltham, MA, US). According to the 

manufacturer’s protocol, cDNA was synthesized from 1.5 µg of total RNA using a mixture of 0.25 µg oligo(dT)20 primer and 0.2 µg of random hexamers with an NG dART RT Kit (Eurx, Gdansk, ´ Poland). The reaction was performed at 25 ◦C for 10 min, followed by 50 ◦C for 50 min. The cDNA was stored at − 20 ◦C. The PCR reaction mixture includes 4 µl of 1/20 diluted cDNA, 0.5 µM gene-specifc primers (Cardenas-Perez et al. (2022) 


DATA-SPECIFIC INFORMATION FOR: Salicornia qPCR SOS1 and NHX1


Number of variables: 6 (salinity treatments)


Number of cases: 4 repetitions for each case


(2024-07-18)
Subject
Earth and Environmental Sciences
Keyword

NHX1, SOS1, gene expression

Related Publication

Salinity-driven changes in Salicornia cell wall nanomechanics and lignin composition. Cárdenas Pérez, S.; Strzelecki, J.; Piernik, A.; Rajabi Dehnavi, A.; Trzeciak, P.; Puchałka, R.; Mierek-Adamska, A.; Chanona Pérez, J.; Kačík, F.; Račko, V.; et al. Environ. Exp. Bot. 2024, 218, 105606 http://repozytorium.umk.pl/handle/item/7033 doi: https://doi.org/10.1016/j.envexpbot.2023.105606

CC0 Creative Commons Zero 1.0 CC0 Creative Commons Zero 1.0

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Salicornia qPCR SOS1 and NHX1.tab
Tabular Data - Original format: MS Excel (XLSX) - 664 B - Jul 18, 2024 - 1 Download
License: CC0 Creative Commons Zero 1.0
10 Variables, 13 Observations - UNF:6:ss+j2orDbNbbyIo/Fsdv4A==
This file contains information related to the qPCR SOS1 and NHX1 in Salicornia
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Readme.txt
Plain Text - 1.9 KB - Jul 18, 2024 - 0 Downloads
MD5: b642932a802c06c995f73a00db705b8f
License: CC0 Creative Commons Zero 1.0
This file contains information related to qPCR data analysis in Salicornia
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