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Piński, Artur, 2025, "Wpływ AIP i PVP na kultury in vitro Fagopyrum tataricum", https://doi.org/10.18150/Z42ISW, RepOD, V1
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Celem badania było zwiększenie zdolności regeneracyjnych kalusa Fagopyrum tataricum poprzez zastosowanie inhibitorów PAL (AIP, AOPP, OBHA) oraz PVP w celu ograniczenia stresu oksydacyjnego i poprawy żywotności tkanek.
Zakres badań obejmował analizę odpowiedzi protoplastów i kompleksów komórek proembriogenicznych na różne stężenia związków. Najlepsze wyniki uzyskano przy 100 µM AIP, które wspierało regenerację roślin oraz obniżało poziom rutyny i stres oksydacyjny. Dodatkowo odnotowano wzrost ekspresji genów embriogenezy somatycznej (LEC2, BBM, WUSCHEL).
Dane te wskazują na korzystny wpływ AIP i PVP na metabolizm i morfogenezę kalusów gryki tatarskiej, co może wspomóc rozwój efektywnych technik kultur in vitro dla roślin użytkowych.
kwas 2-aminoindan-2-fosfonowy, Fagopyrum, kultury in vitro, poliwinylopirolidon (PVP), amoniakoliaza L-fenyloalaniny
Pérez-Pérez, R., Pinski, A., Zaranek, M. et al. Effect of potent inhibitors of phenylalanine ammonia-lyase and PVP on in vitro morphogenesis of Fagopyrum tataricum. BMC Plant Biol 25, 469 (2025). https://doi.org/10.1186/s12870-025-06440-x https://doi.org/10.1186/s12870-025-06440-x doi: 10.1186/s12870-025-06440-x
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