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Chaber, Paweł, 2022, "Research data for the publication 'Surface modification of PHBV fibrous scaffold via lithium borohydride reduction'", https://doi.org/10.18150/MWASK1, RepOD, V1
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Lithium borohydride (LiBH4) reduction was used to modify the surface chemistry of PHBV fibers obtained by electrospinning. The use of LiBH4 allowed the formation of surface hydroxyl groups under very mild conditions, which was crucial for maintaining the fibers’ integrity. The presence of these groups resulted in a noticeable improvement in the surface hydrophilicity of PHBV, as revealed by contact angle measurements. After the treatment with a LiBH4 solution, the electrospun PHBV fibrous mat had a significantly greater number of viable osteoblast-like cells (SaOS-2 cell line) than the untreated mat. Moreover, the results of the cell proliferation measurements correlated well with the observed cell morphology. The most flattened SaOS-2 cells were found on the surface that supported the best cell attachment. Most importantly, the results of the study indicated that the degree of surface modification could be controlled by changing the degradation time and concentration of the borohydride solution.
Redukcję borowodorkiem litu (LiBH4) zastosowano do chemicznej modyfikacji powierzchni włókien PHBV otrzymanych metodą elektroprzędzenia. Zastosowanie LiBH4 umożliwiło tworzenie na powierzchni grup hydroksylowych w łagodnych warunkach, co było kluczowe dla zachowania integralności włókien. Obecność tych grup spowodowała zauważalną poprawę hydrofilowości powierzchni PHBV, co wykazały pomiary kąta zwilżania. Po traktowaniu roztworem LiBH4 elektroprzędzona mata włóknista PHBV miała znacznie większą liczbę żywych komórek osteoblastopodobnych (linia komórkowa SaOS-2) niż mata nie poddana degradacji. Ponadto wyniki pomiarów proliferacji komórek dobrze korelowały z ich morfologią. Najbardziej spłaszczone komórki SaOS-2 znaleziono na powierzchni, która zapewniała najlepsze przyleganie komórek. Co najważniejsze, wyniki badań wykazały, że stopień modyfikacji powierzchni można kontrolować, zmieniając czas degradacji i stężenie roztworu borowodorku.
PHAs, PHBV scaffolds, surface modification, borohydride reduction, electrospinning, rusztowania z PHBV, modyfikacja powierzchni, redukcja borowodorkiem
P. Chaber, G. Tylko, J. Włodarczyk, P. Nitschke, A. Hercog, S. Jurczyk, J. Rech, J. Kubacki, G. Adamus, Surface modification of PHBV fibrous scaffold via lithium borohydride reduction, Materials 2022, 15, 7494, https://doi.org/10.3390/ma15217494 https://doi.org/10.3390/ma15217494 doi: 10.3390/ma15217494
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