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Alterations of fatty acid profiles of different lipid classes in breast cancer - evaluation of their role and diagnostic significance

Version 1.0

Pakiet, Alicja, 2023, "Alterations of fatty acid profiles of different lipid classes in breast cancer - evaluation of their role and diagnostic significance", https://doi.org/10.18150/MHI1FZ, RepOD, V1

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Description

This collection contains the results of studies conducted during realization of the project titled "Alterations of fatty acid profiles of different lipid classes in breast cancer - evaluation of their role and diagnostic significance".

Deposited are:

  • results from GC-MS analysis of fatty acids total lipids obtained using the Folch (Folch J. et al., 1957) in serum and tissues
  • results from GC-MS analysis of fatty acids in fractions obtained by subjecting tissue lipid extract to SPE with method modified from Bodennec (Bodennec J. et al., 2000)
  • results from GC-MS analysis of fatty acids in fractions obtained by subjecting tissue lipid extract to SPE with method modified from Kaluzny (Kaluzny M. et al., 1985)
  • results from colorimetric assays of oxidative stress parameters - lipid peroxidation with TBARS Assay and protein carbonylation in serum and tissues
  • results from quantification of free fatty acids in serum and tissues


Background and objectives of the study

Breast cancer remains a serious health issue globally. Research over the last few decades highlighted the role of lipids in cancer development and progression, as their metabolism is overhauled to satisfy biosynthetic and bioenergetic needs of cancer cells. Fatty acids (FAs) play a central role in lipid metabolism, as the main structural component of most lipid classes. The composition of FAs has multifaceted effects of cell metabolism, they influence membrane properties, affecting the cell-cell metabolism and cell resistance to stress, or rates of energy production via β-oxidation. Enzymes associated with FAs metabolism have been previously shown to be associated with breast cancer course. Our preliminary study of total lipid extracts of breast cancer patients tissues confirmed that cancer tissues have a higher amount of polyunsaturated FAs (present mainly in membrane lipids) and lowered monounsaturated FAs, which are the main component of triacylglycerols, used for energy storage and production. In serum we found the opposite trends. Still, not enough is known about FAs in specific lipid classes (e.g. sphingolipids, ceramides, neutral lipids). All of these classes have important functions in the organism; hence, their FA composition is significant to their function. The main goal of the proposed project was comprehensive profiling of serum and tissues of breast cancer patients, allowing to gain insight into FA composition of different lipid classes. This analysis included profiling of previously unreported or underreported FA groups such as very long-chained FAs, which have potential diagnostic value, or branched-chain and odd-chain FAs, which are reported to have antitumor properties. Also, factors that can correlate with or affect the FA profiles, such as inflammation or oxidative stress were considered.

(2023)
Subject
Medicine, Health and Life Sciences
Keyword

fatty acids, breast cancer, GC-MS, lipids

CC BY - Creative Commons Attribution 4.0 CC BY - Creative Commons Attribution 4.0

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1 to 5 of 5 Files
GC_MS_metadata.ods
Unknown - 49.9 KB - May 12, 2023 - 1 Download
MD5: a122c286325358b9fc5e609589aaaf63
License: CC BY - Creative Commons Attribution 4.0
Tabularized information regarding GC-MS experiments.
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GC_MS_experimental data_tissues_total.ods
Unknown - 52.7 KB - May 12, 2023 - 0 Downloads
MD5: b01cefc5e2602ac5c1c68a7b0d775f0a
License: CC BY - Creative Commons Attribution 4.0
Tabularized data from GC-MS analysis of fatty acids in tissues' total lipid extracts
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GC_MS_experimental data_tissues_fractions.ods
Unknown - 165.2 KB - May 12, 2023 - 0 Downloads
MD5: 1c27d689f1bdf16e9ee03e32bb9a55a2
License: CC BY - Creative Commons Attribution 4.0
Tabularized data from GC-MS analysis of fatty acids in tissues' fractions
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GC_MS_experimental data_serum_total.ods
Unknown - 28.4 KB - May 12, 2023 - 0 Downloads
MD5: 00fc7fc63b6d04c9099cdfe54cf0ad35
License: CC BY - Creative Commons Attribution 4.0
Tabularized data from GC-MS analysis of fatty acids in serum
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biochemical_metadata_experimental data.ods
Unknown - 14.5 KB - May 12, 2023 - 1 Download
MD5: 78c8dded54d0d829ec42f361aae76384
License: CC BY - Creative Commons Attribution 4.0
Tabularized data of free fatty acids concentration, TBARS concentration and protein carbonyl content in serum and tissues
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