Raw data of results obtained during preparation of manuscript entitled: "Functional Hybrid Chitosan Coatings With Cu Nanoparticles on the Shape Memory NiTi Enabling Bactericidal Activity".
Database contains:
- .spm files that are accessible in Gwyddion app,
- .tif files from FIB, SEM, TEM and STEM imaging,
- .tsv files for in situ mass spectrometry files,
- .tif files from optical microscope,
- .tif files from flow cytometry,
- .dpt files from FTIR spectroscopy,
- .xlsx files with ICPMS results (also .ods copie of respective file).
In situ quadrupole mass spectrometer was a part of Inert Gas Condensation supported by magnetron sputtering (Nanogun, Mantis) for nanoparticles deposition.
AFM measurements were conducted using Bruker Dimension ICON XR working in tapping mode with silicon tips on the silicon nitride lever with nominal radii of 8 nm.
SEM measurements were performed using NOVA NANO SEM 200.
The nanoparticle characterization was carried out using transmission electron microscopy Tecnai TF 20 X-TWIN, FEI.
The lamella from CS/CuNPs/CS multilayer was cut using a Focused Ion Beam FEI Nova 200 Nanolab (equipped with a Ga+ beam. Primary, the cutting area was protected with a platinum layer, then the lamella was cut, transferred onto a molybdenum grid, and thinned at −100°C to the final thickness of 64.3 nm)
The thin foil was characterized using Thermo Fisher Scientific Analytical Titan Analítico (HRSTEM, 300 kV) and EDS analysis was performed by an energy dispersive X-ray spectroscopy spectrometer Oxford Instruments Ultim Max TLE 100.
The atomic structure of the coatings was analyzed by Fourier-transform infrared spectroscopy (FTIR, Bruker Tensor II) within the range of 600–4500 cm−1 (80 scans, 4 cm−1 resolution).
The kinetics for Cu release (total mass of ions and NPs) were determined by inductively coupled plasma mass spectrometry (ICP-MS, ELAN 6100 ICP-MS, PerkinElmer Corp., Waltham, MA, USA) after 14 days incubation in Ringer solution (8.6 g/L NaCl, 0.3 g/L KCl, 0.243 g/L CaCl2, pH = 7.15) in static conditions in 37°C.
Flow cytometry (Guava easyCyte) was performed using the Annexin V Apoptosis Detection Kit FITC (Invitrogen) and eBioscience 7-AAD Viability Staining Solution (Thermo Fisher Scientific)
