The dataset contains 32 three-dimensional, cortico-cortical retrograde connectivity patterns revealed by injections of retrograde fluorescent tracers injected in the middle temporal area (MT, or V5) of marmoset monkeys (Callithrix jacchus). Twelve of the animals had lesions placed in the primary visual cortex (V1) in one side of the brain (for details see Yu et al. https://doi.org/10.1523/JNEUROSCI.0188-18.2018). The remaining seven animals received similar tracer injections but no lesions. The dataset also comprises microscopic-resolution (1.9896 µm) images of Nissl- and myelin-stained serial sections, three-dimensional reconstructions of the individual brain hemispheres mapped into a common reference space (Nencki-Monash template, https://doi.org/10.1016/j.neuroimage.2020.117625), various visualizastions and complete set of metadata including, including each animal's age at the times of lesion and tracer injection, sex, hemisphere injected, tracer used, and post-injection survival time.
Tracer injections: Young adult common marmosets received injections of neuronal tracers using established surgical procedures. Under anesthesia and premedication, animals were placed in a stereotaxic frame, and injection sites were selected using stereotaxic coordinates. Projections were identified using fluorescent tracers. Tracers were slowly injected using a microsyringe, and animals received postoperative analgesics. Following typically a 12 to 22 day survival period, the animals were lethally anesthetized and transcardially perfused with heparinized saline and 4% paraformaldehyde. Brains were removed, post-fixed, cryoprotected in sucrose, and cut into coronal sections. One series of sections was mounted unstained for fluorescence microscopy, and the remaining series were stained for Nissl substance, cytochrome oxidase, and myelin using standard protocols. All sections were air-dried and coverslipped. For each hemisphere, every fifth section covering the entire brain was scanned under a Zeiss Axio Imager microscope for the locations of neurons containing the tracers. The locations of labeled neurons relative to section contours were digitised. Attribution of each cell to specific cortical areas and layers was based on comparison with histological sections, followed by computational reconstruction and registration to a reference template following the procedure presented in Majka et al. 2020 (https://doi.org/10.1038/s41467-020-14858-0) study.
The structure of the individual parts of the dataset is detailed in the README.txt file.
(2026-06-01)
