If you don't see your institution, add your dataset to the main dataverse named "RepOD".
Select the dataverse to which you want to add the new dataset:
You need to Sign In/Sign Up to add a dataset.
Share this dataset on your favorite social media networks.
Raza, Sada; Wdowiak, Mateusz, 2025, "Phage-Nanoparticle Cocktails as a Novel Antibacterial Approach: Synergistic Effects of Bacteriophages and Green-Synthesized Silver Nanoparticles", https://doi.org/10.18150/YBVYR9, RepOD, V1
Learn about Data Citation Standards.
Bacteriophages have emerged as promising natural antibacterial agents, offering a targeted approach to combating bacterial infections. While phage-antibiotic cocktails are widely explored to enhance antibacterial efficacy and prevent resistance, research on phage-nanoparticle combinations remains limited. However, antibiotic resistance continues to rise, necessitating alternative strategies. Combining bacteriophages with nanoparticles presents a novel approach that could enhance antibacterial potency while reducing the risk of resistance, yet studies in this area are still scarce. We explore the synergy between green tea extract-capped silver nanoparticles (G-TeaNPs) and bacteriophages in combating pathogenic bacteria (Staphylococcus aureus, Salmonella enterica). G-TeaNPs show no antiphage activity, ensuring compatibility in phage-NP formulations. These combinations significantly reduce bacterial counts in a short time (only 3 hours), e.g., S. aureus survival is around 30% after incubations with just 0.001 mg/mL of G-TeaNPs, with G-TeaNPs and phages alone result in around 80% and 70% survival, respectively. Cytotoxicity tests against eukaryotic 3T3 NIH fibroblast cells confirmed biocompatibility at effective concentrations. Additionally, we examine G-TeaNPs' impact on the free-living protist Acanthamoeba castellanii. Both green tea extract and G-TeaNPs are capable of reducing A. castellanii cell counts by 80%, but only at concentrations larger than 10 mg/mL. Microscopy revealed nanoparticle uptake by amoebae, causing intracellular accumulation and vacuolization, while green tea extract induced similar changes without uptake. Our findings highlight G-TeaNPs as safe, effective agents in phage-nanoparticle antibacterial formulations with dual antimicrobial and amoebicidal properties for therapeutic and environmental applications.
CC0 Creative Commons Zero 1.0
Please select a file or files to be deleted.
The file(s) will be deleted after you click on the Delete button.
Files will not be removed from previously published versions of the dataset.
Please select a file or files to be edited.
For selected file(s) set a license to
Please select a file or files to be downloaded.
Please select a file or files for access request.
Please select restricted file(s) to be unrestricted.
You need to Log In/Sign Up to request access to this file.
Please confirm and/or complete the information needed below in order to continue.
Asterisks indicate required fields
Access to file(s) subject to additional consent under following conditions:
The restricted file(s) selected may not be downloaded because you have not been granted access.
Click Continue to download the files you have access to download.
Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.
Are you sure you want to lift the embargo?
Once you lift the embargo, you will not be able to set it again.
Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.
Use a Private URL to allow those without Dataverse accounts to access your dataset. For more information about the Private URL feature, please refer to the User Guide.
Private URL has not been created.
Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your dataset.
You will not be able to make changes to this dataset while it is in review.
This dataset cannot be published until Institute of Physical Chemistry PAS is published. Would you like to publish both right now?
Once you publish this dataset it must remain published.
Are you sure you want to republish this dataset?
Select if this is a minor or major version update.
This dataset cannot be published until Institute of Physical Chemistry PAS is published by its administrator.
This dataset cannot be published until Institute of Physical Chemistry PAS and RepOD are published.
Are you sure you want to deaccession? The selected version(s) will no longer be viewable by the public.
Contact person for this dataset, having substantive knowledge of the data
Please fill this out to prove you are not a robot.
