The data comprise results obtained using UV–Vis spectroscopy and stopped-flow measurements. They include investigations of Cu(II)–TDMQ20 complex formation and competition for Cu(II) ions between the TDMQ20 ligand and three copper–peptide complexes: Cu(II)–Aβ(1–16), Cu(II)–Aβ(4–16), and Cu(II)–Aβ(5–16).
Main Figures
Figure 1. UV–Vis absorption spectra illustrating the spectrophotometric titration of approximately 20 µM TDMQ20 in 50 mM HEPES buffer (pH 7.4).
Figure 2. Absorption spectra recorded over 1.5 s at 1.5 ms intervals following rapid mixing in a stopped-flow instrument.
Figure 3. Absorption spectra recorded over 40 s at 40.5 ms intervals following the rapid mixing of a solution containing 40 µM Aβ(1–16) and 36 µM CuCl₂ with an equal volume of 40 µM TDMQ20 solution in a stopped-flow instrument.
Figure 4. (A) Absorption spectra recorded over 33 h at 10 min intervals following the mixing of the Cu(II)–Aβ(5–16) complex with a TDMQ20 solution. (B) Absorption spectra recorded over approximately 20 days following the mixing of the Cu(II)–Aβ(4–16) complex with a TDMQ20 solution.
Supplementary Figures
Figure S1. (A) UV–Vis absorption spectra illustrating the spectrophotometric titration of approximately 0.25 mM TDMQ20 in 50 mM HEPES buffer (pH 7.4) with successive aliquots of CuCl₂ at room temperature.
Figure S2. Absorption spectra recorded over 1.5 s at 1.5 ms intervals following the rapid mixing of a solution containing 40 µM TDMQ20 in 100 mM HEPES buffer (pH 7.4) with an equal volume of 36 µM CuCl₂ solution in a stopped-flow instrument.
Figure S3. Absorption spectra recorded over 3 min at 300 ms intervals following the rapid mixing of a solution containing 40 µM TDMQ20 in 100 mM HEPES buffer (pH 7.4) with an equal volume of 36 µM CuCl₂ solution in a stopped-flow instrument.
Figure S4. (A) Absorption spectra recorded over 3 min at 300 ms intervals following the rapid mixing of a solution containing 40 µM TDMQ20 in 100 mM HEPES buffer (pH 7.4) with an equal volume of water in a stopped-flow instrument.
Figure S5. (A) Absorption spectra recorded over 3 min at 300 ms intervals following the rapid mixing of a solution containing 40 µM TDMQ20 and 36 µM CuCl₂ in 100 mM HEPES buffer (pH 7.4) with an equal volume of water in a stopped-flow instrument.
Figure S6. Comparison of the goodness of fit of first-order and second-order kinetic models for Cu(II) transfer from the Cu(II)–Aβ(1–16) complex to TDMQ20.
Figure S7. Absorption spectra illustrating the difference between the expected and experimentally observed efficiencies of Cu(II) extraction by TDMQ20.the rapid mixing of a solution of 40 µM TDMQ20 in 100 mM HEPES buffer (pH 7.4) with an equal volume of 36 µM CuCl2 solution in a stopped-flow instrument
(2026-11)
