The dataset contains research data on the sensitivity of hemocytes in Steatoda grossa spiders to sublethal doses of xenobiotic cadmium and biogenic copper in their diet, considering both short-term and long-term exposure.
The collected data include:
· Immune response analysis – Measurements of the respiratory burst activity of hemocytes after exposure to heavy metal-contaminated food, assessed following the administration of the synthetic immunostimulant phorbol myristate acetate (PMA).
· Antioxidant defense assessment – Levels of selected enzymatic antioxidants, including superoxide dismutase (SOD; EC 1.15.1.1) and catalase (CAT; EC 1.11.1.6), as well as non-enzymatic antioxidants (total antioxidant capacity; TAC) in spider hemolymph in response to chemical exposure, both individually and in combination. Measurements were performed using a Tecan Infinite 200 Microplate Reader.
· Hemocyte ultrastructure evaluation – Light and transmission electron microscopy (TEM) analysis of hemocytes to determine apoptotic, necrotic, or autophagic changes following metal exposure. Instruments used include the Olympus BX60 light microscope, Hitachi H500 transmission electron microscope, and Olympus FluoView FV1000 confocal microscope.
· Metabolic condition comparison – Analysis of hemocyte metabolic status based on exposure duration and the type of metal applied:
· ATP levels and ADP/ATP ratio, assessed using the ApoSENSOR™ ATP Cell Viability Bioluminescence Assay Kit (BioVision, № K254) and ApoSENSOR™ ADP/ATP Ratio Bioluminescence Assay Kit (BioVision, № K255). Measurements were performed with a Tecan Infinite 200 Microplate Reader equipped with a luminometric attachment.
· Quantitative measurement of cell viability in spider hemolymph using flow cytometry with the Annexin V-FITC (fluorescein isothiocyanate) apoptosis detection kit (Abcam®, № ab14085). Analysis was conducted on a Beckman Coulter CytoFLEX flow cytometer using CytExpert Acquisition and Analysis Software (Beckman Coulter, United States).
(2023-07-27)
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