Overweight and obesity are among the most prevalent metabolic disorders and represent one of the major global health challenges. The development of obesity is associated with excessive accumulation of adipose tissue, chronic inflammation, and dysregulation of adipokine secretion, which plays an important role in maintaining the body’s energy homeostasis. In recent years, biologically active peptides involved in the regulation of adipose tissue metabolism have attracted increasing attention. One such peptide is Elabela (Ela), a relatively recently described ligand of the apelin receptor, for which anti-inflammatory and antioxidant effects, as well as an influence on cell proliferation, have been demonstrated. To date, four isoforms of this peptide have been described: Ela-54, Ela-32, Ela-21, and Ela-11, which differ in amino acid length and metabolic activity. Given the preliminary nature of the research, Ela-11 was selected for this project to assess its effects on adipocyte function and adipose tissue metabolism. This topic has not yet been thoroughly characterized and remains largely unexplored in the scientific literature.
The scientific objective of the project was to evaluate the effects of selected concentrations of the Elabela-11 peptide(1, 10, 100, and 1000 nM) on lipolysis and lipogenesis, as well as on the expression and secretion of leptin and adiponectin in isolated rat adipose cells/adipocytes.
Data and file overview:
The dataset consists of 5 files with data collected in the course of the experiment:
1) LIPOLYSIS
2) LEPTIN CONCENTRATION
3) ADIPONECTIN CONCENTRATION
4) LIPOGENESIS
5) mRNA
The files are in .xlsx format
Tested doses of ELA-11 (experimental groups): Control (dose 0 mg/ml); 1 nmol/l; 10 nmol/l; 100 nmol/l; 1000 nmol/l, Insulin and isoproterenol were used as a positive control in some experiemts.
Sharing and access information
All these data are available on license CC-BY.
Abbreviations in files:
Abs
Absorbance
DPM
DPM (Disintegrations Per Minute – rozpady na minutę)
EXP
Experiment
Methodological information:
Isolation of adipocytes from rat perigonadal adipose tissue was performed using the Rodbell method with minor modifications. Briefly, adipose tissue was collected, cleaned of blood vessels and minced with scissors. The tissue was then digested in Krebs–Ringer buffer containing type II collagenase (3 mg/mL) in a shaking water bath. After digestion, the cells were filtered through nylon mesh filters (250 µm), washed four times with warm collagenase-free KRB buffer, counted and divided into experimental groups.
Cells were incubated with different concentrations of Ela-11 for 2 and 6 hours in a shaking water bath at 37°C. After incubation, both the medium and cells were collected for further analyses and stored at −80°C.
Lipolysis intensity was determined based on the amount of glycerol released into the incubation medium using colorimetric and spectrophotometric methods. Secretion of adipokines, including leptin and adiponectin, was measured using commercially available ELISA kits according to the manufacturer’s instructions.
Gene expression analysis of leptin, adiponectin and their receptors at the mRNA level was performed using Real-Time PCR. RNA was isolated from cells using Trizol reagent, and approximately 1 µg of total RNA was reverse-transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit according to the manufacturer’s recommendations. Real-Time PCR reactions were carried out using Sybr Green reagent, species-specific primers and the QuantStudio system (Life Technologies). Relative gene expression levels were calculated using the ΔΔCT method with reference to a housekeeping gene.
Additionally, lipogenesis intensity was assessed using radiolabeled ^14C-glucose and its incorporation into lipids. Lipids were isolated from cells using Dole’s mixture according to the methodology previously described by Kołodziejski et al. (2018).
(2026-06-01)
