This dataset documents analyses of repetitive DNA in Brachypodium species using high-throughput sequencing, bioinformatic approaches, and molecular cytogenetic methods. The study examined the abundance, diversity, evolution, genomic distribution, and chromosomal organization of major repetitive DNA families, including species-specific and shared repeats.
Description of the database:
- Microphotographs (TIF files) of mitotic metaphase chromosomes after fluorescence in situ hybridisation with family 360 bp satellite family as probes. All microphotographs were acquired using a Zeiss AxioImager.Z.2 fluorescent microscope equipped with an AxioCamHMr camera (Zeiss, Oberkochen, Germany). The microphotographs were processed uniformly using ZEN 2.3 Pro (Zeiss).
- Images of Southern Hybridisation of 5S rDNA, centBd and satellite family 1800 bp to DNA isolated from annual and perennial Brachypodium species. Hybridisation signals were documented using ChemiDocXRS (BioRad, Hercules, CA, USA).
- Results of RepeatExplorer2 analyses for perennial Brachypodium species. The repository includes consensus contig sequences generated by RepeatExplorer2 in FASTA format, together with CSV files summarizing the analysis results. The CSV files comprise the results of individual RepeatExplorer2 analyses performed separately for each of the analysed Brachypodium genotypes, as well as the results of a comparative analysis including annual and perennial species. These files provide information on repeat clusters, their classification, abundance, and other associated annotations generated during the RepeatExplorer2 analyses.
For further information, please consult the readme.txt file
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