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Research data for study: Vesicle transfer from stromal to leukemia cells and stroma-mediated protection of leukemia cells from oxidative stress.

Version 1.0

Turos-Korgul, Laura, 2026, "Research data for study: Vesicle transfer from stromal to leukemia cells and stroma-mediated protection of leukemia cells from oxidative stress.", https://doi.org/10.18150/PMTJBS, RepOD, V1

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Description

The files contain research data described in the manuscript entitled: “Stroma-driven horizontal transfer of TCA-related proteins mediates metabolic plasticity and imatinib resistance in chronic myeloid leukemia.”

The dataset includes the analysis of transfer of cellular vesicles from HS-5 bone marrow stromal cells to chronic myeloid leukemia cells (primary patient-derived CD45+ cells isolated from patients’ blood).

Additionally, the dataset includes intracellular reactive oxygen species (ROS) levels measured using the H2DCFDA fluorescent probe. The ROS levels were measured in K562 chronic myeloid leukemia cells, monocultured or co-cultured with HS-5 bone marrow stromal cells. 

The dataset also includes raw western blot images for SOD1 and SOD2, key antioxidant enzymes involved in cellular defense against ROS. The levels were shown in K562 cells, monocultured or co-cultured with HS-5 cells, untreated or treated with imatinib.

The dataset is part of a research project investigating the role of bone marrow stromal cells in metabolic changes in chronic myeloid leukemia cells, funded by the National Science Centre, Poland, under the PRELUDIUM 22 program (grant number 2023/49/N/NZ3/04155).

Subject
Medicine, Health and Life Sciences
Keyword

leukemia, oxidative stress, stroma, vesicles, ROS

Related Publication

Piotr Chroscicki, Nikodem Kasak, Dorota Dymkowska, Laura Turos-Korgul, Dominik Cysewski, Vira Chumak, Dawid Stepnik, Monika Kusio-Kobialka, Agata Kominek, Magdalena Lebiedzinska-Arciszewska, Alicja Krop, Joanna Szczepanowska, Mariusz Wieckowski, Tomasz Stoklosa, Krzysztof Zablocki, Katarzyna Piwocka. Stroma-driven horizontal transfer of TCA-related proteins mediates metabolic plasticity and imatinib resistance in chronic myeloid leukemia. Cell Communication and Signaling. 2025. https://doi.org/10.1186/s12964-025-02564-7 https://doi.org/10.1186/s12964-025-02564-7 doi: 10.1186/s12964-025-02564-7

CC BY - Creative Commons Attribution 4.0 CC BY - Creative Commons Attribution 4.0

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1 to 6 of 6 Files
metadata.txt
Plain Text - 4.3 KB - Apr 24, 2026 - 0 Downloads
MD5: c199f940ea2d29fad90c2f9af9ce7c39
License: CC BY - Creative Commons Attribution 4.0
This file contains explanation for raw and processed data used in the dataset.
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figS3A_processed_data_vesicle_uptake.tab
Tabular Data - Original format: MS Excel (XLSX) - 180 B - Apr 24, 2026 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
5 Variables, 6 Observations - UNF:6:pKK6bzzMk1s1T0jUXWiMDA==
Raw flow cytometry data results correspoding to Supplementary Fig.3A. The dataset contains quantitative analysis of flow cytometry data. Values represent the percentage of CD45+DiD+ cells in co-culture with HS-5 cells.
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figS6A_processed_data_ros_level.tab
Tabular Data - Original format: MS Excel (XLSX) - 228 B - Apr 24, 2026 - 0 Downloads
License: CC BY - Creative Commons Attribution 4.0
6 Variables, 6 Observations - UNF:6:Y7+e/VrQgFO/qpbT57/Kow==
Raw flow cytometry data results correspoding to Supplementary Fig.6A. The dataset contains quantitative analysis. Values represent geometric mean fluorescence intensity (geomean) of H2DCFDA signal measured in manually gated K562 cells in K562 monocultures and co-cultures with HS-5 cells. Normalized values are expressed relative to K562 monoculture (M), which was set to 1.
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figS6B_SOD1_in_K562.TIF
TIFF Image - 71.2 KB - Apr 24, 2026 - 0 Downloads
MD5: d6ab2be8fb05b10fd7bccf8147859954
License: CC BY - Creative Commons Attribution 4.0
Raw Western blot image corresponding to Supplementary Fig.6B. Bands represent SOD1 signal in K562 cells monocultured (mono) and co-cultured with HS-5 cells (co-c), untreated (NT) or treated with 1 uM imatinib (IM).
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figS6B_Tubulin_control_for_SOD1_in_K562.TIF
TIFF Image - 166.6 KB - Apr 24, 2026 - 0 Downloads
MD5: a7b0bb6eadb51b959d9d734a748e45a2
License: CC BY - Creative Commons Attribution 4.0
Raw Western blot image corresponding to Supplementary Fig.6B. Bands represent tubulin signal (loading control for SOD1) in K562 cells monocultured (mono) and co-cultured with HS-5 cells (co-c), untreated (NT) or treated with 1 uM imatinib (IM).
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figS6B_SOD2_and_tubulin_in_K562.TIF
TIFF Image - 95.4 KB - Apr 24, 2026 - 0 Downloads
MD5: 7d0fe593914354d9db6e00d4f2d6608d
License: CC BY - Creative Commons Attribution 4.0
Raw Western blot image corresponding to Supplementary Fig.6B. Bands represent SOD2 and tubulin (loading control for SOD2) signal in K562 cells monocultured (mono) and co-cultured with HS-5 cells (co-c), untreated (NT) or treated with 1 uM imatinib (IM).
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