Metrics
0 Downloads
Share Dataset

Share this dataset on your favorite social media networks.

Detection and prevalence of Epstein-Barr virus (EBV) infection in patients with diffuse large B-cell lymphoma (DLBCL) – raw data

EmbargoVersion 1.0

Jarych, Dariusz, 2026, "Detection and prevalence of Epstein-Barr virus (EBV) infection in patients with diffuse large B-cell lymphoma (DLBCL) – raw data", https://doi.org/10.18150/PD6JKL, RepOD, V1

Learn about Data Citation Standards.

Description

The aim of the scientific activity was to apply sensitive molecular biology methods for the detection and quantification of Epstein–Barr virus (EBV) genetic material in biological samples obtained from patients with diffuse large B-cell lymphoma (DLBCL). 

Raw data generated within the project have been deposited, including:

(i) raw data obtained using droplet digital PCR (ddPCR) for quantitative detection of EBV DNA and RNA in samples from lymphoma patients, targeting the BamHI-W fragment of the EBV genome, with simultaneous detection of the human reference gene RPP30. The dataset also includes ddPCR results for the detection of viral mRNA transcripts of EBER1, LMP1, and EBNA2, analyzed together with the human reference gene GAPDH. The deposited data comprise output data generated by the ddPCR platform;

(ii) microscopic images documentation of the detection of viral EBER1/2 transcripts in tumor tissue from patients with DLBCL and Hodgkin lymphoma (HL), obtained using in situ hybridization (EBER-ISH). The dataset contains raw microscopy PNG images;

(iii) visualization of amplified fragments of the EBV EBNA2 and LMP1 genes obtained by automated capillary electrophoresis using the QIAxcel system in samples from lymphoma patients. The deposited files include graphical outputs (PNG images) showing the separation of PCR products and their corresponding amplicon sizes;

(iv) raw chromatograms (AB1 files) from Sanger DNA sequencing of PCR amplification products of the EBV EBNA2 and LMP1 genes in samples from lymphoma patients. The chromatogram files can be opened and analyzed using, for example, free Chromas software version 2.6.6, available from the Technelysium website.

This study was fully funded by the National Science Centre, Poland (grant no. 2024/08/X/NZ7/01767).

The attached files contain the raw data used for the analyses.

Subject
Medicine, Health and Life Sciences
Keyword

Epstein-Barr virus, lymphomas, viral prevalence and latency

2027-02-28

This dataset is under embargo. Files in this dataset will be available from 2027-02-28.

Delete Dataset

Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.

Delete Draft Version

Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.

Dataset Anonymized Private URL

Use a Anonymized Private URL to allow those without an account in the repository to access your dataset. For more information about the Private URL feature, please refer to the User Guide.

Private URL has not been created.

"WARNING. This dataset has at least one published version. Those who have access to the Anonymized Private URL for this dataset may be able to use its accessible metadata to look up the full, not anonymized version of this dataset.

Dataset Anonymized Private URL

Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your dataset.

Dataset Private URL

Use a Private URL to allow those without an account in the repository to access your dataset. For more information about the Private URL feature, please refer to the User Guide.

Private URL has not been created.

Dataset Private URL

Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your dataset.

Submit for Review

You will not be able to make changes to this dataset while it is in review.

Submit
Publish Dataset

This dataset cannot be published until RepOD is published. Would you like to publish both right now?

Once you publish this dataset it must remain published.

Publish Dataset

Are you sure you want to republish this dataset?

Select if this is a minor or major version update.

Publish Dataset

This dataset cannot be published until RepOD is published by its administrator.

Publish Dataset

This dataset cannot be published until RepOD and are published.

Return for modification


Send a copy of this message to the return e-mail address.
OK
Deaccession Dataset

Are you sure you want to deaccession? The selected version(s) will no longer be viewable by the public.

Embargo

Are you sure you want to lift the embargo?

Once you lift the embargo, you will not be able to set it again.