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Adult young as the fragile ontogenetic stage of the house crickets dietary exposed to GO nanoparticles – digestive enzymes perspective

Version 1.0

Babczyńska, Agnieszka; Tarnawska, Monika; Czaja, Klaudia; Flasz, Barbara; Ajay, Amrendra K.; Napora-Rutkowski, Łukasz; Rozpędek, Katarzyna; Świerczek, Ewa; Kędziorski, Andrzej; Augustyniak, Maria, 2024, "Adult young as the fragile ontogenetic stage of the house crickets dietary exposed to GO nanoparticles – digestive enzymes perspective", https://doi.org/10.18150/P9VLUG, RepOD, V1

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Description

The main goal of this project is elucidation and description of mechanisms of decreased survival (in the first and second generations), and decreased DNA stability (in subsequent generations) after constant exposure of cricket cohorts (Acheta domesticus -model organism) to sublethal amounts of GO in their food, and also evaluation of physiological and biochemical symptoms of adjustment to such stressful condition.

The partgoals of this project are to check and describe:

1. connections between exposure of A. domesticus to GO and composition of gut microbiota and, in turn, their physiology (growth, reproduction and general fitness);

2. microbiomes composition in GO-exposed crickets in relation to strain and generation;

3. microbiome metabolic profiles in GO-exposed crick -dependent scheme;

4. energy budget indices in both strains of GO-exposed crickets in relation to exposure time; digestive enzymes activity;

5. general cell state in GO-exposed crickets in relation to strain and exposure time;

6. effects of GO on telomere length, telomerase activity, DNA methylation and DNAdamage and repair level in successive generations of two house cricket strains

The dataset contains results from chronic graphene oxide intoxication of house cricket. The insects were obtained from two different strains that differ in their ontogenetic development: wild and long-lived. The activity of digestive enzymes in the gut was measured using the API®ZYM test at three insect age stages: larvae, young adults, and mature adults.

(2024-11)
Subject
Medicine, Health and Life Sciences
Keyword

Acheta domesticus, development stages, API®ZYM test

Related Publication

Babczyńska A., Tarnawska M., Czaja K., Flasz B., Ajay A.K., Napora-Rutkowski Ł, Rozpędek K., Świerczek E., Kędziorski A., Augustyniak M. (2024). Adult young as the fragile ontogenetic stage of the house crickets dietary exposed to GO nanoparticles – digestive enzymes perspective. Chemosphere 367: 143641. https://doi.org/10.1016/j.chemosphere.2024.143641 https://doi.org/10.1016/j.chemosphere.2024.143641 doi: 10.1016/j.chemosphere.2024.143641

CC0 Creative Commons Zero 1.0 CC0 Creative Commons Zero 1.0

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API_ZYM_README.txt
Plain Text - 1.1 KB - Nov 19, 2024 - 0 Downloads
MD5: 008caf1699fe94dc258370527fdac4db
License: CC0 Creative Commons Zero 1.0
txt file with description to the API_ZYM.xlsx file
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API_ZYM.tab
Tabular Data - Original format: MS Excel (XLSX) - 24.0 KB - Nov 19, 2024 - 1 Download
License: CC0 Creative Commons Zero 1.0
22 Variables, 144 Observations - UNF:6:Gq4/wI7qB8qwT769a1DSdQ==
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