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Poznan University of Medical Sciences

(Uniwersytet Medyczny im. Karola Marcinkowskiego w Poznaniu)

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Bifunctional silk nanospheres for targeted delivery of oligonucleotide therapeutics to VEGFR-positive cells in the tumor microenvironment

Version 1.0

Florczak-Substyk, Anna; Lorenc, Patryk, 2026, "Bifunctional silk nanospheres for targeted delivery of oligonucleotide therapeutics to VEGFR-positive cells in the tumor microenvironment", https://doi.org/10.18150/MAHNI8, RepOD, V1

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Description

This dataset contains experimental data supporting the publication “Bifunctional silk nanospheres for targeted delivery of oligonucleotide therapeutics to VEGFR-positive cells in the tumor microenvironment.” The study was conducted as part of a research project funded by the National Science Centre (grant no. 2021/43/D/NZ7/00622).

The purpose of the study was to develop and evaluate a targeted drug delivery system based on functionalized silk nanospheres for the selective delivery of oligonucleotide therapeutics to vascular endothelial growth factor receptor (VEGFR)-overexpressing cells in the tumor microenvironment.

The dataset includes data generated during the engineering and characterization of five hybrid silk proteins produced by fusing bioengineered MS1 silk with peptides targeting VEGFR-1 (VE1MS1, VE1bMS1, VE1cMS1) and VEGFR-2 (VE2bMS1, VE2cMS1). These constructs were assessed for receptor binding and cellular internalization in VEGFR-overexpressing cells, resulting in the selection of VE1MS1 and VE2bMS1 as lead candidates for drug delivery system development.

Further data document the formation of bifunctional silk nanospheres obtained by blending VE1MS1 or VE2bMS1 with the nucleic acid–binding silk protein MS2KN. The dataset covers nanoparticle physicochemical characterization, nucleic acid-binding capacity, cytotoxicity, cellular uptake, selective interaction with VEGFR-overexpressing non-small-cell lung cancer cells, and therapeutic efficacy, including siRNA-mediated gene silencing.

The dataset provides comprehensive experimental evidence demonstrating that incorporation of VEGFR-targeting enhances receptor-specific cellular interactions, and, in combination with MS2KN, enables efficient and targeted delivery of siRNA therapeutics to the VEGFR-overexpressing cells of the tumor microenvironment.

Subject

Medicine, Health and Life Sciences

Keyword

silk nanoparticles, cancer therapy, tumor microenvironment, VEGFRs, targeted drug delivery, siRNA

License

CC BY - Creative Commons Attribution 4.0

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		Fig1.zip ZIP Archive - 1.5 MB - Jan 9, 2026 - 0 Downloads MD5: 35b62bd9bf975c8e6a46d375889658b6 License: CC BY - Creative Commons Attribution 4.0	Download
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		Fig6.zip ZIP Archive - 199.0 MB - Jan 9, 2026 - 0 Downloads MD5: ff12fc937b45ad8dd231dc868bca6868 License: CC BY - Creative Commons Attribution 4.0	Download
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		Fig10.zip ZIP Archive - 146.5 KB - Jan 9, 2026 - 0 Downloads MD5: 4d1a0ca3119521fd0fafc908f34ac09b License: CC BY - Creative Commons Attribution 4.0	Download
		SupplFig2.zip ZIP Archive - 8.8 MB - Jan 9, 2026 - 0 Downloads MD5: ceca56fa2723186f18a1894ee7cac801 License: CC BY - Creative Commons Attribution 4.0	Download

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