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University of Gdańsk

(Uniwersytet Gdański)

Uniwersytet Gdański

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Human 20S activators based on the C-terminus of the yeast Blm10 protein

Version 1.0

Jankowska, Elżbieta, 2024, "Human 20S activators based on the C-terminus of the yeast Blm10 protein", https://doi.org/10.18150/LSM0AT, RepOD, V1

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Description

The data collected relate to the study of the properties of analogs of Blm-pep peptide (KYFTGSKLWRSYYA), which proved to be human 20S proteasome activator. These analogs enabled gathering information on the requirements of the human 20S proteasome relative to the structure of its monovalent small-molecule activators.

(2024-02-14)

Subject

Chemistry

Keyword

proteasome, activator

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			Original Format Archival Format (.tab)
		readme.txt Plain Text - 12.1 KB - Feb 15, 2024 - 0 Downloads MD5: b4e34abacb3505db9badb1161744fd2c License: CC0 Creative Commons Zero 1.0	Preview Download
		Blm analogs_names and sequences.pdf Adobe PDF - 53.1 KB - Feb 15, 2024 - 0 Downloads MD5: 00074d973ed8d6988c87bcd2aac52605 License: CC BY - Creative Commons Attribution 4.0 Sequences and assigned compound names	Preview Download
		HPLC and MS data.pdf Adobe PDF - 902.4 KB - Feb 15, 2024 - 0 Downloads MD5: 65a2435641c39830329e777a01e36efa License: CC BY - Creative Commons Attribution 4.0 High-performance liquid chromatography (HPLC) chromatograms and mass spectrometry (MS) spectra confirming the identity and purity of the obtained compounds	Preview Download
		Blm activity on isolated h20S_ChTL TL DabEDS.tab Tabular Data - Original format: MS Excel (XLSX) - 39.1 KB - Feb 15, 2024 - 0 Downloads License: CC BY - Creative Commons Attribution 4.0 25 Variables, 259 Observations - UNF:6:Rc7wkJtWVSbHQNs6XlaEBg== The results of the activity studies of peptidases of the isolated, human 20S proteasome: chymotrypsin-like (ChTL) and trypsin-like (TL), in the presence of Blm-pep analogs. These results were obtained using Suc-LLVY-AMC, and Boc-LLR-AMC proteasome substrates, respectively. The stimulatory abilities of the modulators were also evaluated using a longer FRET-type substrate: Lys(Dabcyl)-Met-Ser-Gly-Phe-Ala-Ala-Thr-Ala-Glu(EDANS)-Gly, abbreviated as DabEDS.	Preview Original File Format (MS Excel (XLSX)) Tab-Delimited RData Format Variable Metadata Data File Citation RIS EndNote XML BibTeX
		Blm activity on isolated h20S_CL.xlsx MS Excel (XLSX) - 182.3 KB - Feb 15, 2024 - 0 Downloads MD5: df7b09beb77ca0ca47923d81ed5dac94 License: CC BY - Creative Commons Attribution 4.0 The results of the activity studies of caspase-like (CL) peptidase of the isolated, human 20S proteasome in the presence of Blm-pep analogs. These results were obtained using Z-LLE-AMC proteasome substrate.	Download
		kinetics of h20S activation by compounds 17 and 18.xlsx MS Excel (XLSX) - 117.8 KB - Feb 15, 2024 - 0 Downloads MD5: 832264ca3233536ca7ad5ecf5338980f License: CC BY - Creative Commons Attribution 4.0 Results of a study of the kinetics of the ChTL peptidase substrate (Suc-LLVY-AMC) degradation by the isolated human 20S proteasome in the presence of the selected, most effective activators	Download
		aggregation propensity of Blm analogs.tab Tabular Data - Original format: MS Excel (XLSX) - 721 B - Feb 15, 2024 - 0 Downloads License: CC BY - Creative Commons Attribution 4.0 4 Variables, 23 Observations - UNF:6:32YBJlueZSIRZ6X86FbnlA== Results of the studies of aggregation tendencies of selected Blm-pep analogs, assessed by thioflavin T (ThT) assay	Preview Original File Format (MS Excel (XLSX)) Tab-Delimited RData Format Variable Metadata Data File Citation RIS EndNote XML BibTeX
		degradation of modulators by h20S.tab Tabular Data - Original format: MS Excel (XLSX) - 9.9 KB - Feb 15, 2024 - 0 Downloads License: CC BY - Creative Commons Attribution 4.0 30 Variables, 66 Observations - UNF:6:C3awxx0wRL1t7vFjFciHZA== Results of a study using analytical ultra-high performance liquid chromatography (UHPLC) of the susceptibility of selected Blm-pep analogs to be digested by the isolated human 20S proteasome	Preview Original File Format (MS Excel (XLSX)) Tab-Delimited RData Format Variable Metadata Data File Citation RIS EndNote XML BibTeX
		protein degradation_aSynuclein.xlsx MS Excel (XLSX) - 419.5 KB - Feb 15, 2024 - 0 Downloads MD5: bfd4a14b700918190dbac87ad085cef6 License: CC BY - Creative Commons Attribution 4.0 Results of testing the ability of Blm-pep analogs to stimulate the isolated human 20S proteasome to digest alpha-synuclein	Download
		protein degradation_Enolase2.xlsx MS Excel (XLSX) - 946.5 KB - Feb 15, 2024 - 0 Downloads MD5: e5fc0a8dc8593e205d4823a612965479 License: CC BY - Creative Commons Attribution 4.0 Results of testing the ability of Blm-pep analogs to stimulate the isolated human 20S proteasome to digest enolase 2	Download

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