Metrics
0 Downloads
Share Dataset

Share this dataset on your favorite social media networks.

Ammonia suppresses the antitumor activity of natural killer cells and T cells by decreasing mature perforin

Version 1.0

Poręba, Marcin, 2026, "Ammonia suppresses the antitumor activity of natural killer cells and T cells by decreasing mature perforin", https://doi.org/10.18150/LMMLWS, RepOD, V1

Learn about Data Citation Standards.

Description

These data summarize Western blot experiments assessing whether human recombinant perforin-1 is processed by cathepsins B, L, S and V under acidic and neutral pH conditions, and by granzyme B under several buffer conditions. The results suggest that perforin-1 shows protease-dependent processing/degradation patterns, with cathepsin effects varying by enzyme concentration and pH, while granzyme B–mediated processing appears buffer- and enzyme-source dependent. 

Subject
Chemistry
Keyword

perforin-1, cathepsins, cathepsin B, cathepsin L, cathepsin S, cathepsin V, granzyme B, proteolytic processing, protease-mediated cleavage, Western blot, pH-dependent proteolysis, cytotoxic lymphocytes, NK cells, T cells, immune effector proteins.

Related Publication

Joanna Domagala, Tomasz M. Grzywa, Iwona Baranowska, Magdalena Justyniarska, Ryan Tannir, Agnieszka Graczyk-Jarzynka, Aleksandra Kusowska, Maria Lecka, Marcin Poreba, Klaudyna Fidyt, Katsiaryna Marhelava, Zofia Pilch, Lea K. Picard, Tomasz Wegierski, Kamil Jastrzebski, Marta Krawczyk, Marta Klopotowska, Monika Granica, Doris Urlaub, Szymon Hajduk, Alexandra Neeser, Spencer Moros, Pawel Kozlowski, Malgorzata Bobrowicz, Marta Miaczynska, Leyuan Ma, Carsten Watzl, Magdalena Winiarska, 2025, Ammonia suppresses the antitumor activity of natural killer cells and T cells by decreasing mature perforin, Cancer Research, 85, 13, 2448–2467 https://doi.org/10.1158/0008-5472.CAN-24-0749 doi: 10.1158/0008-5472.CAN-24-0749

CC BY - Creative Commons Attribution 4.0 CC BY - Creative Commons Attribution 4.0

1 File
PERFORIN_summary.pdf
Adobe PDF - 384.6 KB - Jul 5, 2026 - 0 Downloads
MD5: 917c94a9aa839dcabeb1f1c10bd260b7
License: CC BY - Creative Commons Attribution 4.0
cathepsins cleave perforin
Preview
Download
Select File(s)

Please select a file or files to be deleted.

Delete Files

The file(s) will be deleted after you click on the Delete button.

Files will not be removed from previously published versions of the dataset.

Select File(s)

Please select a file or files to be edited.

Select File(s)

Please select a file or files to be edited.

Select File(s)

Please select a file or files to be edited.

Edit license

For selected file(s) set a license to

Select File(s)

Please select a file or files to be downloaded.

Select File(s)

Please select a file or files for access request.

Select File(s)

Please select restricted file(s) to be unrestricted.

Request Access

  You need to Log In/Sign Up to request access to this file.

Continue
Dataset Terms

Please confirm and/or complete the information needed below in order to continue.

Asterisks indicate required fields

Request Access

Access to file(s) subject to additional consent under following conditions:

Accept
Package File Download
Restricted Files Selected

The restricted file(s) selected may not be downloaded because you have not been granted access.

Restricted Files Selected

The restricted file(s) selected may not be downloaded because you have not been granted access.

Click Continue to download the files you have access to download.

Delete Dataset

Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.

Embargo

Embargo

Are you sure you want to lift the embargo?

Once you lift the embargo, you will not be able to set it again.

Delete Draft Version

Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.

Dataset Anonymized Private URL

Use a Anonymized Private URL to allow those without Dataverse accounts to access your dataset. For more information about the Private URL feature, please refer to the User Guide.

Private URL has not been created.

"WARNING. This dataset has at least one published version. Those who have access to the Anonymized Private URL for this dataset may be able to use its accessible metadata to look up the full, not anonymized version of this dataset.

Dataset Anonymized Private URL

Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your dataset.

Dataset Private URL

Use a Private URL to allow those without Dataverse accounts to access your dataset. For more information about the Private URL feature, please refer to the User Guide.

Private URL has not been created.

Dataset Private URL

Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your dataset.

Submit for Review

You will not be able to make changes to this dataset while it is in review.

Submit
Publish Dataset

This dataset cannot be published until Wrocław University of Science and Technology is published. Would you like to publish both right now?

Once you publish this dataset it must remain published.

Publish Dataset

Are you sure you want to republish this dataset?

Select if this is a minor or major version update.

Publish Dataset

This dataset cannot be published until Wrocław University of Science and Technology is published by its administrator.

Publish Dataset

This dataset cannot be published until Wrocław University of Science and Technology and RepOD are published.

Return for modification


Send a copy of this message to the return e-mail address.
OK
Deaccession Dataset

Are you sure you want to deaccession? The selected version(s) will no longer be viewable by the public.