The following files include data presented in the manuscript “Efficient multiphoton microscopy with picosecond laser pulses,” written by Katarzyna Kunio, Jakub Bogusławski, and Grzegorz Soboń.
Figure 2a – optical spectrum of the picosecond oscillator measured directly at the oscillator output and its spectral phase
Figure 2b – output pulse autocorrelation measured directly at the oscillator output together with Gaussian fit function
Figure 3a_1 – autocorrelation of the picosecond pulse with a Gaussian fit measured at the laser system output (after AOM)
Figure 3a_2 – autocorrelation of the picosecond pulse with a Gaussian fit measured at the the microscope’s sample plane
Figure 3b – comparison of the reconstructed temporal profile of the femtosecond pulse at the laser system output (after AOM) and in the microscope’s sample plane.
Figure 4a – TPE fluorescence images of convallaria majalis sample obtained using femtosecond pulse train (scale bar: 75 µm): 227 fs, 15 MHz, 0.7 mW.
Figure 4b – TPE fluorescence images of convallaria majalis sample obtained using picosecond pulse train (scale bar: 75 µm): 10 ps, 0.3 MHz, 0.7 mW.
Figure 4c – SHG images of urea microcrystals using femtosecond pulse train (scale bar: 200 µm): 227 fs, 15 MHz, 0.3 mW.
Figure 4d – SHG images of urea microcrystals using picosecond pulse train (scale bar: 200 µm): 10 ps, 0.3 MHz, 0.3 mW.
Figure 4e – pixel intensity histograms of images shown in Fig. 4a and b (convallaria majalis sample, TPE fluorescence).
Figure 4f – pixel intensity histograms of images shown in Fig. 4c and d (urea microcrystals, SHG).
Figure 5a – TPE fluorescence images of convallaria majalis sample obtained using femtosecond pulse train (scale bar: 75 µm): 227 fs, 15 MHz, 0.7 mW.
Figure 5b – TPE fluorescence images of convallaria majalis sample obtained using picosecond pulse train (scale bar: 75 µm): 10 ps, 1.1 MHz, 0.7 mW.
Figure 5c – SHG images of urea microcrystals using femtosecond pulse train (scale bar: 200 µm): 227 fs, 15 MHz, 0.3 mW.
Figure 5d – SHG images of urea microcrystals using picosecond pulse train (scale bar: 200 µm): 10 ps, 1.1 MHz, 0.3 mW.
Figure 5e – pixel intensity histograms of images shown in Fig. 5a and b (convallaria majalis sample, TPE fluorescence).
Figure 5f – pixel intensity histograms of images shown in Fig. 5c and d (urea microcrystals, SHG).
(2024-07)
