This dataset contains the results of a study on the effect of nickel on the midgut of the shrimp Neocaridina davidi.
During the experiment, the shrimp were divided into experimental groups, exposed to nickel, and returned to clean water to verify whether the changes were reversible.
The animals were divided into the following experimental groups:
CT – control – the animals stayed in clean water throughout the experiment;
Ni 1:0 and Ni 2:0 – shrimps bred in the aquarium water with the addition of Ni for 1 and 2 weeks respectively;
Ni 1:1 and Ni 1:2 – shrimps after a week’s cultivation in the aquarium water with the addition of Ni and returned to clean water and cultured for 1 and 2 weeks respectively;
Ni 2:1 and Ni 2:2 – shrimps after 2 weeks of cultivation in the aquarium water with the addition of Ni and returned to clean water for 1 and 2 weeks respectively.
The changes in the midgut, consisting of the intestine and the hepatopancreas, were analyzed.
Attached are 4 zip files:
1) Confocal microscopy - DHE,
2) Transmision Electron Microscopy,
3) Flow cytometry - MUSE_oxidative stress,
4) TXRF - total-reflection X-ray fluorescence spectrometry.
Confocal microscopy - DHE - Confocal Microscope: Olympus FluoView FV1000, Magnification: 60, Staining: DHE + DAPI.
Description: Dihydroethidium (DHE) staining.
Dihydro- ethidium (hydroethidine) is a dye commonly used to assess the location of reactive oxygen species (ROS) accumulation in cells and tissues (differentiation of ROS+ and ROS− cells). Isolated organs (intestine and hepatopancreas) were incubated with 30 μM of DHE (Invitrogen) and DAPI staining (1 μg/ml, 30 min, room temperature (RT), in the dark) and analyzed with an Olympus FluoView FV1000 confocal microscope with argon lasers: 405 nm laser (detection of DAPI) and 559 nm (detection of TMR). The result: blue fluorescence of nuclei and red fluorescence of localization of ROS.
Transmision Electron Microscopy - Hitachi H500, Counterstaining
Flow cytometry - MUSE_oxidative stress - Flow cytometer=Muse® Cell Analyzer
Test=Muse® Oxidative Stress Kit (Merck Millipore, № MCH100111)
Description=The Muse® Oxidative Stress Kit (Merck Millipore, № MCH100111) was used for the quantitative analysis of cell populations undergoing oxidative stress.
It distinguishes two types of cell populations: live cells, which are ROS(−), and cells exhibiting ROS (ROS+ cells).
TXRF - total-reflection X-ray fluorescence spectrometry - Spectrometer=S4 T-STAR equipped with a 50 W power Mo target X-ray tube with a multilayer monochromator and an SSD detector.
The X-ray tube operated at 50 kV and 1000 µA.
The measurements were conducted under ambient air conditions, with a counting time of 1000 seconds, and Y applied as the internal standard.
Description=This method allows measuring the concentration of nickel accumulated in shrimp.
Three repetitions were performed for each group.
The abbreviations used: <LOD and SD mean: below the limit of detection (<LOD) and standard deviation (SD).
The files were converted to open files to facilitate access and subsequent use of the data (.ods, .tif and .jpg).
(2025)
