In this study, the structure of the anodic bacterial community was analyzed through high-throughput sequencing techniques. Specifically, amplicon sequencing of the V3-V4 region of the 16S rRNA gene was employed to characterize the microbial populations. The dataset contains the nucleotide sequences of the 16s rRNA gene of 10 samples. Samples came from anodic chambers of microbial fuel cells. Five of them were supplemented with glucose and five with benzene and glucose.
The amplification and library preparation for sequencing utilized specific primer sequences, 341F and 785R, which are well-suited for targeting the V3-V4 hypervariable regions of the 16S rRNA gene. Polymerase chain reaction (PCR) was conducted using the Q5 Hot Start High-Fidelity 2X Master Mix. The reaction conditions adhered to the manufacturer's guidelines, ensuring high accuracy and efficiency in the amplification process.
Sequencing was performed on the Illumina MiSeq platform in paired-end (PE) mode, with each read being 300 nucleotides in length (2x300nt), facilitated by the Illumina v3 kit. This approach allows for comprehensive coverage and high-resolution insights into the bacterial community structure.
Preliminary analysis of the sequencing data was conducted automatically using the MiSeq Reporter (MSR) software version 2.6, integrated into the MiSeq sequencer. This initial data processing step helped to streamline subsequent bioinformatic analyses, enabling the identification and characterization of the bacterial taxa present in the samples.
Description of samples attached below.
(2024)
