Using coffee husks as bedding material is a sustainable choice that also benefits poultry. A study examined the expression of immune-related genes in the intestinal mucosa and indicator bacteria in the caecal content of broiler chickens bedded with added coffee husks. One-day-old Ross 308 chickens were divided into four groups of 10 birds each in five replicates: C, the control group kept on wheat straw bedding; CH10, a group bedded with 10% coffee husks and 90% wheat straw; CH25, a group bedded with 25% husks and 75% straw; and CH50, a group bedded with 50% husks and 50% straw. After 42 days, the birds were slaughtered, and the caecal mucosae were removed for RNA isolation, while the caecal content was collected for bacterial DNA isolation. The study analyzed the expression of genes involved in intestinal immune response and host organism defense, as well as the relative abundance of indicator bacteria.
The dataset presents raw data related to the publication: Biesek J., Dunisławska A., Kozdruń W. 2024. The effect of coffee husks used as pellet bedding material on the intestinal barrier, immune-related gene expression and microbiota composition in the broiler chicken caecum. Journal of Veterinary Research, 68(3), 443-449. https://doi.org/10.2478/jvetres-2024-0053.
The data was obtained as a part of a project entitled "Research on the use of coffee husks in bedding for broiler chickens and ducks", No. 2021/05/X/NZ9/00820, financed by the National Science Center (Poland). (Project Investigator: Biesek, Jakub). The samples were taken from mentioned project.
This dataset contains data on:
- bacteria dataset
- gene expression (mucosa) dataset
(2024-03-28)
