This study investigates the post-polyploidization evolution of repetitive DNA in Brachypodium hybridum genotypes of different ages and origins. While LTR retrotransposons showed largely additive inheritance, tandem repeats were highly dynamic. The dataset documents comparative analyses of two allotetraploid genotypes and their diploid ancestral species, focusing on retrotransposon copy number, satellite DNA abundance, and the chromosomal organization of repetitive sequences.
Description of the database:
- Microphotographs (TIF files) of mitotic metaphase chromosomes after fluorescence in situ hybridisation (FISH) with CentBd, CentBs satellite sequences and retrotransposons RT (CRM, Athila) conserved domain as probes. All microphotographs were acquired using a Zeiss AxioImager.Z.2 fluorescent microscope equipped with an AxioCamHMr camera (Zeiss, Oberkochen, Germany). The microphotographs were processed uniformly using ZEN 2.3 Pro (Zeiss).
- Results of RepeatExplorer2 analyses for Brachypodium species. The repository includes consensus contig sequences generated by RepeatExplorer2 in FASTA format, together with CSV files summarizing the analysis results. The CSV files comprise the results of individual RepeatExplorer2 analyses performed separately for each of the four Brachypodium genotypes, as well as the results of a comparative analysis including all four genotypes. These files provide information on repeat clusters, their classification, abundance, and other associated annotations generated during the RepeatExplorer2 analyses.
For further information, please consult the readme.txt file