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MK-8776 and Olaparib combination demonstrates lack of adverse effects on liver tissues in ovarian cancer PDX model

Version 1.0

Rogalska, Aneta, 2025, "MK-8776 and Olaparib combination demonstrates lack of adverse effects on liver tissues in ovarian cancer PDX model", https://doi.org/10.18150/CKASLL, RepOD, V1

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Description

Histopathological Analysis of Liver Tissues in olaparib-resistant PDX model treated with PARPi alone or in combination with a CHK1 inhibitor

Objectives:To evaluate the effectiveness and side effects of the drugs in patient-derived xenograft (PDX) models, we examined whether ovarian tumor cells metastasized to the liver after therapy. 

Methods: Olaparib-resistant PDX tumors were treated with PARPi alone or in combination with a CHK1 inhibitor for histopathological analysis. The levels of Ki-67 (a proliferation marker) and CHK1 kinase phosphorylation at Ser345 were assessed. Hematoxilin and eosin staining (H&E) staining was used to differentiate and visualize the detailed structure of tissue components. 

Results: An Hematoxilin and eosin (H&E) based evaluation of the liver tissue from mice with olaparib-resistant ovarian cancer revealed no evidence of metastasis to the liver. Ki-67 and pCHK staining showed no changes in liver tissue, indicating the absence of metastases and confirming that the treatment did not induce hepatotoxic effects.

The dataset includes microscopic images from IHC and H&E staining of liver tissues.

Folder and file list: 

Folder 1. Hematoxilin and eosin staining of liver tissue images (png format) and staining procedure description (odt format).

Folder 2. Immunohistochemistry staining of liver tissue images_Ki67 marker (png format) and preparation of liver sections and immunohistochemistry staining procedure description (odt format).

Folder 3. Immunohistochemistry staining of liver tissue images_pCHK1 marker (png format) and preparation of liver sections and immunohistochemistry staining procedure description (odt format).

Readme file. Data&file overview, methodological information, environmental/experimental conditions, abbreviations used to describe images

 

Subject
Medicine, Health and Life Sciences
Keyword

liver tissue, ovarian cancer, metastasis, targeted therapy, olaparib, CHK1 inhibitor

Related Publication

Bębenek, W.; Gajek, A.; Marczak, A.; Malý, J.; Smejkal, J.; Statkiewicz, M.; Rusetska, N.; Bryś, M.; Rogalska, A. MK-8776 and Olaparib Combination Acts Synergistically in Hepatocellular Carcinoma Cells, Demonstrating Lack of Adverse Effects on Liver Tissues in Ovarian Cancer PDX Model. Int. J. Mol. Sci. 2025, 26, 834. https://doi.org/10.3390/ijms26020834 https://doi.org/10.3390/ijms26020834 doi: 10.3390/ijms26020834

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