If you don't see your institution, add your dataset to the main dataverse named "RepOD".
Select the dataverse to which you want to add the new dataset:
You need to Sign In/Sign Up to add a dataset.
Share this dataset on your favorite social media networks.
Witkowska Piłaszewicz, Olga, 2025, "Immunological responses to tetanus and influenza vaccination in donkeys", https://doi.org/10.18150/CB6TAU, RepOD, V1
Learn about Data Citation Standards.
The dataset concerns the immunological response of donkeys (Equus asinus) to a combined vaccination against tetanus and equine influenza. The data were collected in an experimental study aimed at assessing temporal changes in T and B lymphocyte populations as well as monocyte subpopulations using flow cytometry. This dataset enables evaluation of cell-mediated immune responses in donkeys—a species for which such data are extremely limited in the literature—making it a unique contribution to the fields of veterinary and comparative immunology.
The dataset consists of raw tabular result summaries (xlsx, ods). These files document the percentage and mean fluorescence intensity (MFI) of selected immune cell subsets (e.g., CD4+, CD8+, FoxP3+, CD5+, PanB, CD14+) at three time points: pre-vaccination, 30 days after the initial dose, and 30 days after the booster dose. The .csv files also contain demographic information (age, sex) and metadata related to sample collection (date, individual ID).
Data acquisition was performed using a BD FACSCanto II flow cytometer and analyzed with Kaluza 1.5 software. Peripheral blood mononuclear cells (PBMCs) were isolated using SepMate™–Lymphoprep™ kits. In vitro stimulation was carried out using a PMA/ionomycin cocktail with protein transport inhibitors. Antibodies used in this study were selected based on confirmed cross-reactivity with Equus asinus antigens.
donkeys, tetanus, flow cytometry, influenza
CC BY - Creative Commons Attribution 4.0
Please select a file or files to be deleted.
The file(s) will be deleted after you click on the Delete button.
Files will not be removed from previously published versions of the dataset.
Please select a file or files to be edited.
For selected file(s) set a license to
Please select a file or files to be downloaded.
Please select a file or files for access request.
Please select restricted file(s) to be unrestricted.
You need to Log In/Sign Up to request access to this file.
Please confirm and/or complete the information needed below in order to continue.
Asterisks indicate required fields
Access to file(s) subject to additional consent under following conditions:
The restricted file(s) selected may not be downloaded because you have not been granted access.
Click Continue to download the files you have access to download.
Are you sure you want to delete this dataset and all of its files? You cannot undelete this dataset.
Are you sure you want to lift the embargo?
Once you lift the embargo, you will not be able to set it again.
Are you sure you want to delete this draft version? Files will be reverted to the most recently published version. You cannot undelete this draft.
Use a Private URL to allow those without Dataverse accounts to access your dataset. For more information about the Private URL feature, please refer to the User Guide.
Private URL has not been created.
Are you sure you want to disable the Private URL? If you have shared the Private URL with others they will no longer be able to use it to access your dataset.
You will not be able to make changes to this dataset while it is in review.
This dataset cannot be published until RepOD is published. Would you like to publish both right now?
Once you publish this dataset it must remain published.
Are you sure you want to republish this dataset?
Select if this is a minor or major version update.
This dataset cannot be published until RepOD is published by its administrator.
This dataset cannot be published until RepOD and are published.
Are you sure you want to deaccession? The selected version(s) will no longer be viewable by the public.
Contact person for this dataset, having substantive knowledge of the data
Please fill this out to prove you are not a robot.
