Article title: Age- and lifespan-dependent differences in GO caused DNA damage in Acheta domesticus.
Abstract: The rising applicability of graphene oxide (GO) should be preceded by detailed tests confirming its safety and no toxicity. Sensitivity to GO of immature or with different survival strategies individuals has not been studied so far. Therefore, in the present research, we focused on the GO genotoxic effects, examining selected parameters of DNA damage (Total DNA damage, double-strand breaks - DSB, 8-hydroxy-2 '-deoxyguanosine - 8-OHdG, abasic site - AP sites), DNA damage response parameters, and global methylation in the model organism Acheta domesticus. Special attention was paid to various life stages and lifespans, using wild (H) and selected for longevity (D) strains. DNA damage was significantly affected by stage and/or strain and GO exposure. Larvae and young imago were generally more sensitive than adults, revealing more severe DNA damage. Especially in the earlier life stages, the D strain reacted more intensely/inversely than the H strain. In contrast, DNA damage response parameters were not significantly related to stage and/or strain and GO exposure. Stage-dependent DNA damage, especially DSB and 8-OHdG, with the simultaneous lack or subtle activation of DNA damage response parameters, may result from the general life strategy of insects. Predominantly fast-living and fast-breeding organisms can minimize energy-demanding repair mechanisms.
The article is written within the project "Modern nanoparticle and model organism - a toxic relation? Physiological and cellular causes of developmental disorders in Acheta domesticus after oral exposure to graphene oxide"
The main goal of this project is elucidation and description of mechanisms of decreased survival and decreased DNA stability after constant (five subsequent generations) exposure of cricket cohorts (Acheta domesticus -model organism) to sublethal amounts of GO in their food, and also evaluation of physiological and biochemical symptoms of adjustment to such stressful condition.
The partgoals of this project are to check and describe:
1. connections between exposure of A. domesticus to GO and composition of gut microbiota and, in turn, their physiology (growth, reproduction and general fitness);
2. microbiomes composition in GO-exposed crickets in relation to strain and generation;
3. microbiome metabolic profiles in GO-exposed crick -dependent scheme;
4. energy budget indices in both strains of GO-exposed crickets in relation to exposure time; digestive enzymes activity;
5. general cell state in GO-exposed crickets in relation to strain and exposure time;
6. effects of GO on telomere length, telomerase activity, DNA methylation and DNA damage and repair level in successive generations of two house cricket strains
(2022-12-19)
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